复方参七汤对人结肠癌细胞株裸鼠肝转移影响_临床医学论文.docVIP

复方参七汤对人结肠癌细胞株裸鼠肝转移影响_临床医学论文 复方参七汤对人结肠癌细胞株裸鼠肝转移影响_临床医学论文 作者：陈瑞新,徐青,于秀,陈玉泉,沈洪薰 　[摘要]　　目的:建立人结肠癌细胞株裸鼠肝转移模型，研究复方参七汤对裸鼠肝转移模型的影响。方法:采用BALB/C裸鼠，Ls174t人大肠癌细胞株, 脾脏切除脾脏种植法建立裸鼠肝转移模型。给予复方参七汤颗粒饲料喂养，观察体态及肿瘤生长情况；采用巢式逆转录聚合酶链反应（RT-PCR）检测裸鼠循环血中CK20 mRNA表达；标本作病理组织学检查。结果:①脾脏切除脾脏种植法建立裸鼠结肠癌肝转移模型的成功率为100%，病理组织学证实肝转移癌细胞呈人结肠癌低分化腺癌特征；②实验组裸鼠肿瘤数目及重量明显低于对照组，0.05；③实验组裸鼠肝转移血中CK20 mRNA表达为0.398±0.143,明显低于对照组0.518±0.090，0.05。结论:复方参七汤可显著降低肝转移裸鼠血中CK20 mRNA表达，抑制裸鼠结肠癌肝转移的发生及发展，可作为大肠癌术后预防复发和肝转移治疗的手段之一。 　　[关键词]　　结肠癌;肝转移;裸鼠;CK20 mRNA;RT-PCR 　　Abstract: Objective To establish the liver metastases of colonic adenocarcinoma for experimental study of the effect of compound shenqitang in nude mice.Methods Nude mice liver metastasis model of colonic cancer was established with human colonic cancer cells line (Ls 174t) inoculated into mice spleen.Granular forage with compound shenqitang was fed in experimental group and granular forage without compound shenqitang was fed in control group daily. The weight and size of the mice and growth of the carcinoma were recorded.All specimens were examined histologically.CK20 mRNA in blood in nude mice with liver metastasis of colonic carcinoma was detected with nested RT-PCR. Results The incidence of liver metastasis was 100 % in this intrasplenic injection model. The pathological results showed that tumour cells of liver metastases was poorly differentiated human colonic adenocarcinoma.In experimental group, the tumor number and the weight of liver metastases were significantly lower than those in control group(0.05).Using semiquantitative examination,in experimental group the relative value of CK 20 mRNA expression in blood (0.40±0.14) was significantly lower than that(0.52±0.09) in control group (0.05).Conclusion Compound shenqitang could effectively inhibit the occurrence of liver metastasis carcinoma and decrease the positive expression of CK20 mRNA in experimental group,suggesting that compound shenqitang may become the potential therapeutic strategy for liver metatst