大鼠实验性胃溃疡自愈期间胃黏膜组织凋亡相关因子Bax,Bcl2的表达变化_临床医学论文.docVIP

大鼠实验性胃溃疡自愈期间胃黏膜组织凋亡相关因子Bax,Bcl的表达变化_临床医学论文 大鼠实验性胃溃疡自愈期间胃黏膜组织凋亡相关因子Bax,Bcl的表达变化_临床医学论文 作者：张静 陈丽薇 吕洋 吴靖芳 【摘要】 目的：探讨大鼠实验性胃溃疡自愈期间促凋亡蛋白Bax与抗凋亡蛋白Bcl在胃黏膜组织中表达的变化。方法：胃前壁黏膜下注射冰乙酸制备大鼠胃溃疡模型，免疫组织化学法检测正常组、溃疡组、盐水组大鼠胃黏膜组织Bax和Bcl表达变化,并应用图像分析系统软件进行积分光密度值(IOD)分析。结果：Bax阳性细胞在胃溃疡组1d即增多，表达强度强，显著高于正常组与相应盐水组(0.01);之后随术后时间延长,阳性细胞逐渐减少,表达强度渐弱,至术后23d，溃疡组Bax IOD与对照组相比差异无统计学意义(Pgt;0.05)。Bcl阳性细胞随术后时间延长表达逐渐增多,至胃溃疡损伤后的第6天Bcl阳性细胞数量最多，表达强度最强，胃溃疡损伤后10d及14d Bcl略低于溃疡后6d，但仍维持于较高水平，至胃溃疡损伤后23d，Bcl显著降低，与正常组、盐水组相比差异无统计学意义(Pgt;0.05)。结论:大鼠胃溃疡自愈期间Bax与Bcl共同参与了胃黏膜的损伤修复。在胃溃疡损伤早期以促凋亡因素为主，抗凋亡因素对溃疡胃黏膜的保护及修复作用主要是在损伤后的6～14d。 【关键词】 胃溃疡;Bcl免疫组织化学;大鼠 　　【ABSTRACT】 Objective:To investigate the expression of Bax and Bclinduced ulcers were caused by injection acetic acid to submucosal of paries anterior gastricus.The expressions of Bax and Bcl2 in gastric ulcer group(GU),normal saline group(NS)and normal group(N)were detected by immunohistochemical method,and the results of IOD were analyzed by using the image analytical systems.Results:Bax positive cells significantly increased and showed strong expression on the first day after the experimental gastric ulcer in rats,the IOD value was markedly higher than that in control groups(0.01).Then the IOD values of Bax cells in GU group were gradually decreased,and on 23rd day，which had not statistical difference compared with that of the control groups(Pgt;0.05).The expression of Bcl2 gradually increased and the IOD of Bcl2 reached the peak(IOD=21.038±7.537)on the 6th day in GU group,which showed the reduction tendency,but on the 10th and 14th days,the IOD values still stayed at the higher level.On the 23th day in GU group,IOD decreased significantly,and had no statistical difference compared with that of control groups(Pgt;0.05).Conclusion:Bax and Bcl2 proteins participate in renovating the gastric mucosa during healing of experimental gastric ulcer in rats.The proapoptosis protein Bax plays main role in the early stage of the experimental gastric ulcer,and antiapotosis prot