大鼠岛叶与杏仁核电点燃癫痫模型的相关性研究_临床医学论文.docVIP

大鼠岛叶与杏仁核电点燃癫痫模型的相关性研究_临床医学论文 大鼠岛叶与杏仁核电点燃癫痫模型的相关性研究_临床医学论文 作者：刘庆祝，王峰，牟青春，张培松，徐文中 【摘要】 目的 电刺激Sprague-Dawley大鼠岛叶、杏仁核及同时刺激岛叶、杏仁核建立复杂部分性癫痫模型，探讨岛叶癫痫的可能发病机制。方法 健康雄性SD大鼠60只，随机分成三组：岛叶点燃组(IK)、杏仁核点燃组(AK)、岛叶杏仁核同时点燃组(BK)。分别将双极电极植入左侧岛叶、左侧杏仁核和同时植入岛叶杏仁核；测定后放阈后每日给予两次电刺激，刺激参数：强度为后放阈1.5倍，频率60 Hz，波宽1 ms，持续时间1 s。结果 岛叶组和杏仁核组后放阈值差异有统计学意义(P＜0.05)；三组点燃时间差异有统计学意义，岛叶组点燃最快，岛叶杏仁核同时点燃组点燃最慢(P＜0.05)；三组点燃率无明显差异。结论 岛叶是一个癫痫症状发作区；岛叶癫痫的发生、发展与杏仁核点燃存在差异；同时点燃表现为点燃的拮抗作用。 【关键词】 岛叶；杏仁核；点燃；癫痫模型 　　Abstract: Objective To stimulate Spragueate insular and amygdale simultaneously and to establish Complicated Partial Epilepsy Model for investigating possible nosogenesis of Insular Epilepsy. Methods 60 male SpragueDawley Rats were divided into 3 groups randomly: Insular kindling group (IK), Amygdale kindling group (IK), Insular and amygdale simultaneously kindling group (BK). Bipolar electrodes were implanted into left Insular or left Amygdale or both and rats were stimulated at 1.5 times of afterdischarge threshold twice daily. Stimulus train consisted of 1ms (square biphasic) pulses at 60 Hz, with train duration of 1s. Results There was significant difference in the aftercharge threshold between insular and amygdale kindling groups (P＜0.05). There was significant deviation at kindling speed among three groups: IK was the most rapid kindling group and BK group was kindled more slowly (P＜0.05). There was no significant difference in kindling rate among three groups. Conclusion Insular is an area that causes epilepsy symptom. The genesis and development of Insular Epilepsy are different from Amygdale Epilepsy and it shows antagonism to kindling. 　　Key words: insular; amygdale; kindling; epilepsy model 　　癫痫是常见的脑部发作性疾病，而岛叶癫痫由于位置深在、诊断困难、机制不清等原因成为最难处理的一类癫痫。岛叶在癫痫(特别是颞叶癫痫)中的作用，在国际上已逐渐被一些学者重视［1］。而在国内,除少量有关岛叶占位性病变引起癫痫发作的病例报道外,此方面的研究报道很少。电点燃模型是研究颞叶癫痫最理想的模型，它通过反复刺激特定脑区导致其癫痫发作活性不断增强， 最终形成稳定的癫痫发作状态［2］。本研究试图通过电刺激Sprague-Dawley大鼠岛叶、杏仁核及同时刺激岛叶杏仁核建立复杂部分性癫痫动物模型，观察三者在后放阈、点燃时间和触发阈值上的差异，并分析出现此种差异的可能原因，探讨岛叶在癫痫中的发病机制。 　　1 材料和方法 　　1. 1