大鼠心脏多巴胺1受体与心肌肥厚的关系_临床医学论文.docVIP

大鼠心脏多巴胺1受体与心肌肥厚的关系_临床医学论文 大鼠心脏多巴胺1受体与心肌肥厚的关系_临床医学论文 作者：高嵩丹，王瑾，赵荣瑞，支建明 【摘要】 目的 采用腹主动脉狭窄建立大鼠心肌肥厚模型，观察大鼠心肌肥厚时心肌组织多巴胺1受体（DA1-R）反应性的变化，借以阐明心脏DA1-R与心肌肥厚的关系。方法 实验分5组：假手术对照组（C组）、腹主动脉狭窄组（A组）、腹主动脉狭窄+倍他乐克组（A+B组）；腹主动脉狭窄+倍他乐克+SCH23390组(A+B+S组)；假手术+倍他乐克+FODA组（B+F组）。各组均于4周后测定大鼠心功能和左心室/体重比值（LVW/BW）；另外还测定了A组心肌肥厚大鼠的心肌组织在FODA作用后cAMP生成量的变化。结果 （1）A+B组各指标变化与A组无明显差异；（2）A+B+S组的LVW/BW与心肌细胞横截面积的明显减小，与A组或A+B组相比差异均有显著性（0.05）；（3）B+F组LVW/BW明显加大，形成了一定程度的心肌肥厚，与对照组相比0.05。（4）A组大鼠心肌组织在FODA作用后，其cAMP的生成量明显高于对照组（0.05）。结论 心脏DA1-R参与了病理情况下心肌肥厚的形成，由压力超负荷引起心肌肥厚时，心脏DA1-R的反应性增高。 【关键词】 心肌肥厚; 多巴胺受体; fenoldopam; SCH23390 【Abstract】 Objective The aim of this study is to examine the relations of cardiac dopamine-1 receptors （DA1-R）to the development of myocardial hypertrophy. Methods The myocardial hypertrophy model was established by ligating the abdominal aorta.Thirty Wistar healthy male rats were randomly divided into five groups: aorta constriction group (group A); sham-operation control group (group C ); aorta constriction + β-adrenoreceptor blocker (betaloc) group (group A+B); aorta constriction + betaloc + SCH23390 group (group A+B+S); normal rats + betaloc + FODA group (group B+F). Cardiac function and left ventricular weight/body weight（LVW/BW）were determined for all groups at 4 weeks after treatment. In addition, the production of cAMP induced by FODA stimulation in hypertrophied myocardium (group A) was also examined. Results It was shown that: (1) There were no significant differences for all parameters between group A+B and group A. (2) In group A+B+S, the changes of LVW/BW and the cross section area of cardiac myocyte were all significantly attenuated as compared with group A or group A+B (0.05). (3) LVW/BW of B+F group were greatly increased as compared with control group (0.05); (4) The cAMP production of hypertrophied myocardium (in group A) induced by FODA stimulation was greatly higher than that of control group (0.05).Conclusion It is suggested that cardiac DA1-R are involved in the formation