大鼠无血预充体外循环伴心脏停跳模型的建立_临床医学论文.docVIP

大鼠无血预充体外循环伴心脏停跳模型的建立_临床医学论文 大鼠无血预充体外循环伴心脏停跳模型的建立_临床医学论文 作者：宋丹丹，高光洁，孙莹杰，张铁铮，周锦，张瑜，姚婧 【摘要】 目的 摸索建立大鼠无血预充体外循环伴心脏停跳模型的实验方法。 方法 以雄性SD大鼠为实验动物，经右颈静脉引流、右股动脉灌注建立体外循环模型。采用6%羟乙基淀粉进行无血预充，经右颈动脉穿刺置入冷灌管至主动脉根部，正中劈胸骨，阻断主动脉，灌注心脏停搏液使心脏停跳，15 min后开放主动脉，心脏自主复跳，体外循环1 h后停转流。停转流2 h后大鼠血压心率稳定视为模型成功。结果 模型死亡率5%(2/40只)。心脏自主复跳成功率为94.7%(36/38只)。34只顺利停转流，模型成功率85%。结论 本实验建立的无血预充大鼠体外循环伴心脏停跳模型，操作简单，费用低廉，成功率较高，是研究体外循环期间全身炎症反应、脏器保护机制及评估相关保护措施的良好实验工具。 【关键词】 大鼠;体外循环;心脏停跳;无血预充;动物模型 Establishment of a Rat Model of Cardiopulmonary Bypass withCardioplegic Arrest Primed without Blood 　　Abstract: OBJECTIVE To establish a rat model of the cardiopulmonary bypass (CPB) with cardioplegic arrest primed without blood. METHODS Adult male Sprague-Dawley rats (400～500g, n = 40) were used for all experiments. Blood was drained from the right atrium via jugular vein catheter into the CPB circuit and return to the right femoral artery. The miniature cardiopulmonary bypass was primed with 6% hetastarch without blood. A catheter through the right carotid artery was inserted into the aortic root for administration of cardioplegic solution. After median sternotomy aortic arch was clamped. Cardioplegic arrest was accomplished by a cardioplegic solution. After 15 min of cardioplegia, the aortic cross-clamping was released. CPB was weaned after 1 hr. It is a validation criteria of the animal model that the arterial blood pressure and heart rate of rats were stable at 2 hrs post CPB. RESULTS Mortality rate was 5 percent. The rate of heart spontaneous re-beating was 94.7 percent. Blood pressure and heart rate were stable in 34 of 40 rats at 2 hrs post CPB. Success rate of model was 85 percent. CONCLUSION The rat model of CPB with cardioplegic arrest primed without blood is easy to be performed with low cost and higher success rate. This model is suitable for the investigation of the pathophysiological changes concerning CPB-related multiple organ dysfunction and possible protective interventions. 　　Key words： Rat; Cardiopulmonary B