大鼠离体肺再灌注模型的建立和改进_临床医学论文.docVIP

大鼠离体肺再灌注模型的建立和改进_临床医学论文 大鼠离体肺再灌注模型的建立和改进_临床医学论文 作者：魏以桢， 吴清玉，史世勇 【摘要】 　　目的 本实验利用微创方法建立大鼠离体肺灌注模型。方法 将一只受体大鼠股动脉、颈静脉插管，引流出静脉血灌注低温保存后的供体肺，氧合后的血液回输入受体大鼠体内。每只受体大鼠建立控制性交叉循环后，灌注一只供体肺1 h，取股静脉流出血样测定pH、PO2、PCO2和Hb的变化情况。结果 各组间pH、PO2、PCO2和Hb经统计学分析无明显差异（Pgt;0.05）。结论 控制性交叉循环下受体大鼠血流动力学稳定，能够较长时间地提供符合生理状态的静脉化全血。可用于供体肺保存及缺血再灌注损伤的研究。 【关键词】 动物模型　肺移植　供体　缺血再灌注 　　Establishment and Modification of Isolated Lung Transplantation and Abstract: OBJECTIVE To establish and modify of the isolated lung transplantation and reperfusion model in rats with a relatively less-invasive way. METHODS Angiocatheters were inserted into femoral artery and jugular vein of the recipient rat to establish the controlled cross-circulatory. The venous cannula was connected to the pulmonary artery of the ex vivo lung block after preservation and cold storage to provide inflow，and Pulmonary effluent blood from the lung block was collected and returned to the support rat via the femoral artery for detoxification and deoxygenation. Each support rat was used to perfuse one lung block for at least one hour. The inflow sample to each lung block was analyzed for pH, pO2, pCO2, and Hb to follow alterations in support rat physiology. RESULTS There were no statistical differences in pH, PvO2, PvCO2 or Hb （Pgt;0.05）. CONCLUSION The support rat can deliver constant systemic venous blood at stable physiologic parameters to the ex vivo lung block and this animal model can be used in research of lung transplantation and ischemic-reperfusion injury. Key words： Animal model; Lung transplantation;Donor;Ischemic-reperfusion injury; 目前，肺移植已成为一种治疗多种终末期肺部疾患的有效手段。但早期移植物功能障碍(primary graft failure，PGF)的发生始终是手术失败的主要原因，与移植后并发症发生率及病死率明显相关［1-2］。PGF病因复杂，降低PGF发生率的努力多集中在延长供体肺保存时间以及减轻供体肺缺血再灌注方面。原位大动物肺移植模型操作复杂，技术要求高，维护成本较高，且失败率高，小动物的体外循环(extracorporeal circulation,ECC)模型较少报道［3-4］。因此，建立一种简单可靠、重复性好、费用低廉的动物模型来评价肺保存及再灌注效果非常必要。本实验利用微创方法建立大鼠离体肺灌注模型，为开展大鼠肺移植手术供体肺保存及术后缺血再灌注损伤等研究提供了一种选择。 1 材料与方法 1.1 材料 清洁级纯种健康雄性SD大鼠（北京维通利华实验动物技术有限公司提供，实验动物机构许可证号：scxk(京)2