大黄素和黄芪多糖对实验性肝损伤大鼠肝脏超微结构的影响_临床医学论文.docVIP

大黄素和黄芪多糖对实验性肝损伤大鼠肝脏超微结构的影响_临床医学论文 大黄素和黄芪多糖对实验性肝损伤大鼠肝脏超微结构的影响_临床医学论文 作者：张欣，党双锁，程延安，贾晓黎，陈明霞，刘恩岐 【摘要】 目的 探讨大黄素和黄芪多糖单用及联合应用对肝损伤大鼠肝脏超微结构的影响及意义。方法 采用四氯化碳(CCl4)等复合因素诱导大鼠肝损伤模型。选取清洁雄性SD大鼠90只，随机分为6组：正常对照组(10只)、模型组(20只)、大黄素组(15只)、黄芪多糖组(15只)、大黄素和黄芪多糖联合给药组(15只)、秋水仙碱组(15只)。除正常对照组和单纯造模组外其他各组同时给对应的药物干预，即大黄素40mg/(kg·d)、黄芪多糖200mg/(kg·d)、大黄素40mg/(kg·d)+黄芪多糖200mg/(kg·d)、秋水仙碱0.1mg/(kg·d)，共12周。采集标本后测定肝组织羟脯氨酸(Hyp)含量，电镜观察各组大鼠肝脏的超微结构。结果 大黄素、黄芪多糖单用或联合应用均可以减轻CCl4对大鼠肝脏组织的损伤程度，尤其是联合用药可以明显减少肝组织Hyp含量;保护肝脏的超微结构改变，表现为肝细胞结构接近正常，包膜规整，细胞质内见少量脂滴，线粒体嵴排列规整，内质网增生且明显扩张，肝窦隙可见呈梭形或杆状的肝星状细胞(HSC)，肝窦内充斥少量纤细胶原纤维束，Kupffer细胞脂滴不多。结论 大黄素和黄芪多糖对CCl4致大鼠肝脏的超微结构损伤具有一定的预防保护作用。 【关键词】 大黄素;黄芪多糖;肝损伤;超微结构;大鼠 　　ABSTRACT: Objective To explore the effect and significance of emodin and astragalus polysaccharides (ASP) as well as their combined administration on hepatic ultrastructure in rat hepatic injury. Methods Hepatic injury was induced by multiple factors including carbon tetrachloride (CCl4). Altogether 90 SD rats of clean grade were divided randomly into 6 groups: normal control group (10 rats), model group (20 rats), emodin group (15 rats), ASP group (15 rats), emodinASP combination group (15 rats) and colchicine group (15 rats). The rats were exposed to different concentrations of emodin [40mg/(kg·d)], APS [200mg/(kg·d)], combination drug [emodin 40mg/(kg·d) combined with APS 200mg/(kg·d)] and colchicine [0.1mg/(kg·d)] in parallel by oral gavage (once a day for 12 weeks). At the end of week 12, hydroxyproline (Hyp) content in liver homogenates of all rats were determined. The hepatic ultrastructures of all rats were observed by electron microscope. Results Emodin and APS could ameliorate CCl4induced hepatic injury in rats, especially coadministration of emodin and ASP showed significant prevention of the elevation of Hyp content and protection of hepatic ultrastructure: nearlyormal hepatic cytostructure, regular amicula, manipulus lipid droplet proliferation of intracytoplasm, reduced swelling of mitochondrion, prolif