大黄酚对AD模型小鼠学习记忆的影响_临床医学论文.docVIP

大黄酚对AD模型小鼠学习记忆的影响_临床医学论文 大黄酚对AD模型小鼠学习记忆的影响_临床医学论文 作者：董晓华 张海红 张丹参 张力 丁杰 【摘要】 目的 观察大黄酚对β淀粉样肽（Aβ25）致AD小鼠学习记忆障碍及脑组织过氧化氢（H2O2）含量、过氧化氢酶（CAT）、Na+酶和Ca2+酶活性的影响。方法 给小鼠一次性脑室内注射凝聚态Aβ25（1.0 mmol/L）造AD模型，造模后腹腔注射药物或溶剂，连续注射14 d。采用Y迷宫法测试小鼠学习记忆能力，每天每鼠训练10次，连续测试5 d，以正确反应次数作为观测指标。用比色法测定小鼠脑组织H2O2含量、CAT、 Na+酶和Ca2+酶活性。结果 与对照组相比，模型组正确反应次数降低，脑内H2O2含量升高；CAT、Na+酶和Ca2+酶活性显著降低；与模型组比较，大黄酚各组小鼠正确反应次数从测试2 d起明显增加，小鼠脑内H2O2含量明显降低，而CAT、Na+酶和Ca2+酶活性显著升高。结论 大黄酚对Aβ25致AD小鼠记忆障碍有保护作用，其作用机制可能与抗氧化、改善能量代谢有关。 【关键词】 大黄酚；β淀粉样肽；学习记忆；过氧化氢；过氧化氢酶；ATP酶 【Abstract】 Objective To observe the effects of chrysophanol on abilities of learning and memory, contents of H2O2, activities of catalase (CAT), Na+. Methods The aggregated Aβ2535 3 μl (1 mmol/L) was intracerebroventricularly injected once into mice to make AD model. The solvent was administered by ip for control and model group, while chrysophanol (10.0,1.0,0.1 mg/kg) was injected for chrysophanol groups for 14 days. The abilities of learning and memory in mice were observed through Ymaze test for 5 days, in which every mouse was disciplined 10 times every day and the number of correct response was as observed indicators. Then the contents of H2O2 and the activities of CAT, Na+K+ATPase and Ca2+ATPase in brain tissue in mice were detected through colorimetry. Results Compared with control group, the number of correct response was decreased，the contents of H2O2 were increased, and activities of CAT, Na+re higher than in model group significantly. Conclusions Chrysophanol shows protective effects on learning and memory impairment in AD mice induced by Aβ2535. Possible mechanisms are involved in the actions of antioxidation and improving energy metabolism. 【Key words】 Chrysophanol；β；Learning and memory；H2O2；catalase；ATPase 　　阿尔茨海默病(AD)是一种较常见的神经系统退行性疾病，其主要临床特征为记忆减退和认知功能障碍，β淀粉样蛋白在脑内沉积形成老年斑是AD的发病基础。近年来研究表明大黄酚能改善衰老动物学习记忆障碍〔1〕，降低大鼠脑内过氧化脂质含量〔2〕，提示抗氧化作用可能是其抗衰老作用的机制之一，但大黄酚抗氧化作用的具体作用环节及对脑组织能量代谢的影响尚未见报道。本研究采用一次性直接侧脑室注射凝聚态β淀粉样肽（Aβ25）的方法建立AD小鼠模型，进一步探讨大黄酚改善学习记忆作用的机制。 　　1 材料与方法