大黄虫丸对阿霉素肾硬化大鼠血栓素B2、6-酮-前列腺素F1α的影响_临床医学论文.docVIP

大黄虫丸对阿霉素肾硬化大鼠血栓素B2、6-酮-前列腺素F1α的影响_临床医学论文 大黄虫丸对阿霉素肾硬化大鼠血栓素B2、6-酮-前列腺素F1α的影响_临床医学论文 作者：陈继红,孙伟,高坤,周栋,刘丽,赖仁胜,朱萱萱 【摘要】 目的 探讨大黄虫丸对阿霉素肾硬化大鼠尿蛋白排泄率、血脂、血浆血栓素B2(TXB2)和6-酮-前列腺素F1α(6-Keto-PGF1α)的影响。方法 大鼠在单侧肾切除、分次阿霉素尾静脉注射的基础上喂饲高脂饲料,造成弥漫性系膜增生伴局灶节段性肾小球硬化动物模型。将大鼠分为假手术组、空白组、大黄虫丸组、苯那普利组,观察各组大鼠尿蛋白、血脂、血浆TXB2和6-Keto-PGF1α水平。结果 大黄虫丸可明显降低大鼠尿蛋白排出量；降低血浆胆固醇含量；升高血浆6-Keto-PGF1α及降低TXB2水平。结论 大黄虫丸能够祛瘀扶正,促进气血运行,通畅血脉,改善血流动力学,对阿霉素所致的肾损害具有一定的保护作用,其机制可能与其调节血栓素/前列腺素平衡有关。 【关键词】 大黄虫丸；血栓素B2；6-酮-前列腺素F1α；大鼠 　　Abstract：Objective To observe the effect of Dahuangzhechongwan on protein excretion, serum cholesterol, TXB2 and 6-keto-PGF1α in rats with adriamycin-induced nephrosclerosis. Methods The rats received twice-intravenous injections of adriamycin after one kidney was removed, and were fed with hyperlipide food to establish an animal model for diffusion mesangioproliferation following with focal segmental glomerulosclerosis. The rats were randomly divided into shame-operated group, adriamycin nephrosclerosis group, Dahuangzhechongwan group, benazepril group. Urinary protein excretion, blood fat, TXB2 and 6-keto-PGF1α were observed. Result Proteinuria extract was decreased, the plasma cholesterol was significantly lower, the level of TXB2 was cut down and 6-Ket-PGF1α was increased by Dahuangzhechongwan. Conclusion Dahuangzhechongwan has the role of simutianeous application of elimination and tonification, promoting the circulation of Qi and blood, eliminating blood stasis, improving the hemodynamics. Dahuangzhechongwan has the renal protection on rat with adriamycin-induced nephrosclerosis, the mechanism may be relate with adjusting the balance of TXB2 and 6-keto-PGF1α. 　　Key word：Dahuangzhechongwan；TXB2；6-keto-PGF1α；rat　　大黄虫丸出自《金匮要略·血痹虚劳病脉症并治第六》,乃医圣张仲景所创,其方构思精巧,以大黄荡下逐瘀、虫破血通络为君；配以水蛭、虻虫、蛴螬活血通络、攻逐瘀血,合以桃仁、干漆增强活血祛瘀之功；佐以黄芩清热,杏仁利气,芍药、地黄、甘草、白蜜滋阴润燥养血；酒服以行药势为使药。该方融补虚扶正、清利祛邪、活血逐瘀为一体,方中药物配伍适合慢性肾小球疾病肾虚湿瘀这一病理机制,我们用之于临床,对延缓肾功能衰竭的进展取得了良好的疗效。在此基础上,引入阿霉素肾硬化大鼠模型,进一步探讨其作用机制。 　 　　1 材料与方法 　　1.1 动物和饲料 健康雄性SD大鼠,周龄8～12周,体重200～