大鼠一侧睾丸扭转后切除对另侧睾丸组织的保护作用_临床医学论文.docVIP

大鼠一侧睾丸扭转后切除对另侧睾丸组织的保护作用_临床医学论文 大鼠一侧睾丸扭转后切除对另侧睾丸组织的保护作用_临床医学论文 作者：王浩，秦乐，黄渊，陈肖鸣 【摘要】 　　目的 ：探讨大鼠一侧睾丸扭转后行切除术对另侧睾丸组织的保护作用。方法：将63只青春期前SD大鼠随机分为假手术组、扭转复位组和扭转切除组，每组21只。假手术组将左侧阴囊打开分离暴露睾丸后，不予扭转，即将睾丸再固定于阴囊壁上，并缝合阴囊。扭转复位组和扭转切除组则依Turner法建立左侧睾丸扭转模型，均先扭转4 h，其后前者行复位固定术，后者行切除术。三组分别于复位后4 h、24 h、1周各处死大鼠7只，取右侧睾丸行丙二醛（MDA）、白介素-6（IL-6）检测及病理评估，左侧睾丸仅行病理评估。结果 ：各组左侧睾丸病理评分值均达到最差分数，病理切片结果示存在绝大部分坏死或完全性坏死。与扭转切除组比较，扭转复位组对侧睾丸的MDA、IL-6值、病理评分等均有不同程度升高，且1周时生精细胞层数结合精细小管直径所示的预后情况亦较差；而各指标在扭转切除组与假手术组的相比中未示明显差异。结论 ：单侧睾丸扭转复位后对侧睾丸早期即可出现继发性损伤。在明确扭转侧睾丸出现坏死后，不复位直接行切除术可以保护对侧睾丸。 【关键词】 一侧睾丸扭转 对侧睾丸损伤 睾丸切除术 保护 　　Abstract: Objective: To explore the protective effects of orchiectomy on contralateral testis of SD rats with unilateral testicular torsion through the biochemical and histological indexes. Methods ：Sixty-three prepubertal Sprague-Dawley rats were randomly divided into 3 groups， with 21 rats in each group. The sham operation group underwent a sham operation of the left testis under general anaesthesia. The torsion/detorsion group and the torsion/orchiectomy group underwent a similar operation but the left testis was rotated 720 °clockwisely for 4 h， maintained by fixing the testes to the scrotum，then detorsion was performed in the torsion/detorsion group，while orchiectomy was performed in the torsion/orchiectomy group. Both testes of seven rats from each group were sacrificed at 4 hour，24 hour and 1 week intervals after surgery. Then，the biochemical parameters such as malondialdehyde (MDA) and interleukin-6 (IL-6) were evaluated ；histology index and seminiferous tubule diameter were assessed on right testes，while the left were only assessed by histology index. Results:Compared with The torsion/orchiectomy group，the biochemical parameters and histological grades in the torsion/detorsion group increased in different degrees， and the prognosis status demonstrated by the number of spermatogenic cell associated with the decreased mean diameter of the seminiferous tubule， there are a worse prognoses in the torsion/