大肠癌中Ezrin蛋白的表达及其与临床病理特征的关系_临床医学论文.docVIP

大肠癌中Ezrin蛋白的表达及其与临床病理特征的关系_临床医学论文 大肠癌中Ezrin蛋白的表达及其与临床病理特征的关系_临床医学论文 作者：范婕　史威 李秀娟 郭颖 白美玲 【摘要】 目的：探讨Ezrin在大肠癌中的表达特点及其与临床病理参数的关系。方法：应用免疫组织化学S法检测100例大肠癌组织及11例癌旁正常黏膜中Ezrin的表达情况。结果：Ezrin阳性表达率在大肠正常粘膜、大肠癌组织中分别为36.4%、75%,差异有统计学意义(0.05);淋巴结转移组Ezrin阳性率(89.6%)显著高于无淋巴结转移组(61.5%)(0.05);在大肠癌中,Ezrin异常表达与大肠癌患者的年龄、性别、肿瘤的发生部位以及分化程度无关(Pgt;0.05)。结论：Ezrin表达水平提高提示癌组织具有更高的浸润、侵袭能力和淋巴结转移风险，检测 Ezrin有望成为判断大肠癌患者预后的新指标或治疗肿瘤转移的一个靶点。 【关键词】 肠肿瘤;肿瘤转移;免疫组织化学;膜细胞骨架连接蛋白 Ezrin,a member of the ezrinleton linker and is involved in cellular functions,including epithelial cell morphogenesis and adhesion[1].It is encode by vil2(6q25.2～q26)and its functional domain is FERM domain.The inactivation of Ezrin causes a massive cell retraction and leads to the destruction of both cellcell and cellsubstrate adhesion,whereas the overexpression of Ezrin in insect cells results in enhanced cell adhesion[2,3].The expression of Ezrin in a series of patients with colorectal carcinoma was studied,using immunohistochemistry to elucidate its functional importance and clinical significance. 　　1 Materials and methods 　　1.1 Materials 　　Between March 2005 and May 2006,100 colorectal carcinoma samples were obtained from the archives of the First Affiliated Hospital,Hebei North University Zhangjiakou,which were all surgically resected without chemotherapy and radiotherapy before operation.There were 41 men(41%)and 59 women(59%)with a median age of 58.3 years(range,30～78 years).Forty patients in rectum.Fortyeight patients(48%)had histologically confirmed lymph node metastasis,whereas the remaining 52 patients(52%)were found to have no clinical or histopathologic evidence of lymph node involvement.According to current World Health Organization classification,52 patients were well differentiated,26 patients were moderately differentiated,11 patients were poorly differentiated and 11 patients were Mucous.Immunohistochemistry. 　　1.2 Methods 　　The samples were fixed in 10% neutrally buffered formalin and paraffin embedded,processed