大肠癌中MTA1和HIF-1α的表达与淋巴管生成的关系_临床医学论文.docVIP

大肠癌中MTA1和HIF-1α的表达与淋巴管生成的关系_临床医学论文 大肠癌中MTA1和HIF-1α的表达与淋巴管生成的关系_临床医学论文 作者：杨珍玉 周序珑 夏明汗 蒋光喻 钟雪云 杜彬 【摘要】 【目的】 探讨大肠癌中转移相关基因1(MTA1)、缺氧诱导因子-1α(HIF-1α)蛋白的表达及其与淋巴管生成的关系。【方法】 用免疫组织化学SP法检测81例大肠癌中MTA1、HIF-1α蛋白的表达及D2-40标记的淋巴管密度。【结果】 81例大肠癌中MTA1、HIF-1α阳性表达率分别为66.7%和63%，MTA1和HIF-1α的表达均与大肠癌Duke?蒺s分期和淋巴结转移相关(P lt; 0.05)而与组织学分级和浸润深度无关(P gt; 0.05)，大肠癌中MTA1和HIF-1α蛋白表达呈正相关(P lt; 0.05)，MTA1和HIF-1α蛋白过表达病例肿瘤旁淋巴管密度分别为18.4 ± 4.9、18.0 ± 5.3，高于MTA1和HIF-1α不表达和弱表达病例(15.6 ± 4.5、15.4 ± 4.2)，两者的差异有统计学意义(P lt; 0.05)。【结论】 MTA1和HIF-1α蛋白的过表达可能促进了大肠癌旁淋巴管的生成，在肿瘤淋巴结的转移中起一定作用。 【关键词】 结直肠癌; MTA1; HIF-1α; 淋巴管密度 　Abstract： 【Objective】 To investigate the expression of metastasis-associated gene 1 (MTA1)， hypoxia inducible-factor l alpha (HIF-1α) in human colorectal cancer and to explore the relationship between their expression and lymphangiogenesis. 【Methods】 The expression of MTA1 and HIF-1α， and D2-40 marked lymphatic vessel density (LVD) were detected by immunohistochemical staining in eighty-one human colorectal cancer cases. 【Results】 The positive rates of MTA1 and HIF-1α expression were 66.7% and 63.0 % respectively in the eighty-one human colorectal cancer cases. The expression of MTA1 and HIF-1α were both correlate with Duke’s stage and lymph node metastasis (P lt; 0.05) but did not correlate with the pathological grade and depth of invasion (P gt; 0.05). In addition， MTA1 and HIF-1α expression has a positive correlation in human colorectal cancer (P lt; 0.05). In MTA1 and HIF-1α over-expression cases， the peritumoral LVD was 18.4 ± 4.9 and 18.0 ± 5.3 respectively and was higher than that of the MTA1 and HIF-1α expression none or weak cases (15.6 ± 4.5， 15.4 ± 4.2)， the difference has statistically significance (P lt; 0.05). 【Conclusion】 The overexpression of MTA1 and HIF-1α in colorectal cancer possibly promote the cancer lymphangiogenesis and play a role in lymph node metastasis. 　　]淋巴道转移是大肠癌转移的重要途径，而淋巴管的生成则是促进大肠癌淋巴道转移的主要因素。MTA1(metastasis-associated gene 1)是近年来发现的一个与肿瘤转移密切相关的癌基因，MTA1可促进大肠癌侵袭转移，但其与淋