大肠癌中β-catenin和E-cadherin的表达及其临床意义_临床医学论文.docVIP

大肠癌中β-catenin和E-cadherin的表达及其临床意义_临床医学论文 大肠癌中β-catenin和E-cadherin的表达及其临床意义_临床医学论文 作者：戴文斌，任占平，陈蔚麟，杜娟，石吉吉，唐德艳【摘要】 目的 探讨β-catenin和E-cadherin在大肠癌发生、发展中的作用。方法 应用免疫组织化学方法检测30例正常大肠粘膜、30例大肠腺瘤、10例大肠腺瘤恶变及50例大肠癌组织中β-catenin和E-cadherin蛋白的表达情况。结果 大肠癌、大肠腺瘤恶变和大肠腺瘤β-catenin胞浆和(或)胞核异位表达率显著高于正常大肠粘膜（P均lt;0.01），大肠癌β-catenin异位表达率显著高于大肠腺瘤（0.01）。大肠癌中β-catenin、E-cadherin膜表达缺失率显著高于大肠腺瘤和正常大肠粘膜（P均lt;0.01），且与大肠癌组织分化程度、浸润深度、淋巴结转移、Dukes分期有关。结论 β-catenin异位表达与大肠癌的发生有关，是大肠癌发生的早期事件；β-catenin、E-cadherin膜表达缺失与大肠癌的侵袭、转移有关。 【关键词】 大肠；肿瘤；β-连接素；E-钙粘素；免疫组织化学 　　Abstract： Objective To investigate the roles of beta-catenin and E-cadherin play in the carcinogenesis and progression of colorectal carcinoma. Methods The expression and beta-catenin and E-cadherin proteins were detected by immunohistochemistry in 30 cases of normal colorectal mucosa，30 cases of colorectal adenoma，10 cases of malignant colorectal adenoma and 50 cases of colorectal carcinoma tissues. Results The ectopic expression rates of beta-catenin and E-cadherin proteins in cell nucleus and cytoplasm of colorectal carcinoma，malignant colorectal adenoma and colorectal adenoma were all significantly higher than that of normal colorectal mucosa (0.01).The ectopic expression rate of beta-catenin in colorectal carcinoma was higher than the colorectal adenoma (0.01).Compared to colorectal adenoma and normal mucosa，loss of membranous beta-catenin，E-cadherin expression rate in colorectal carcinoma were higher，there was statistically significant difference (0.01)，and the reduced membranous beta-catenin and E-cadherin expression were closely related with the differentiation degree，the invasive depth，the metastasis and Dukes stage in colorectal carcinoma tissues. Conclusion The cytoplasmic and nuclear beta-catenin expression may play a pivotal role in carcinogenesis and progression of colorectal carcinoma.Loss of membranous beta-catenin and E-cadherin expression may be related to the invasion and metastasis of colorectal carcinoma. 　　Key words： large int