大黄虫丸对肺纤维化模型肺泡灌洗液中超氧化物歧化酶及脂质过氧化物的影响_临床医学论文.docVIP

大黄虫丸对肺纤维化模型肺泡灌洗液中超氧化物歧化酶及脂质过氧化物的影响_临床医学论文 大黄虫丸对肺纤维化模型肺泡灌洗液中超氧化物歧化酶及脂质过氧化物的影响_临床医学论文 作者：李瑞琴，李伟，宋建平，张瑞，张现伟 【摘要】 目的探讨大黄虫丸防治肺纤维化作用及机理。方法用平阳霉素诱导肺纤维化模型大鼠后，用药组每天灌胃大黄虫丸，分别于第7天，14天后处死各组大鼠，观察、比较各组大鼠肺部病理组织学改变及肺泡灌洗液中超氧化物歧化酶(SOD)、脂质过氧化物(LPO)表达。结果第14天模型对照组出现一定程度的肺纤维化，模型对照组肺纤维化程度显著重于正常对照组（0.05），大黄虫丸组肺纤维化程度显著低于模型对照组（0.05）；第7天、第14天模型对照组SOD活性显著低于正常对照组（0.05）；大黄虫丸组与模型对照组相比无显著差异（Pgt;0.05）。第7天模型对照组LPO活性与正常对照组相比无显著差异（Pgt;0.05）；第14天模型对照组LPO显著高于正常对照组（0.05）；大黄虫丸组显著低于模型对照组（0.05）。结论大黄虫丸能明显减轻平阳霉素所致的大鼠纤维化程度，并能显著降低LPO的表达。 【关键词】 肺纤维化； 超氧化物歧化酶； 脂质过氧化物； 大黄虫丸； 大鼠 　　Abstract：ObjectiveTo investigate the effect and mechanism of Dahuangzhechong (DHZC) on pulmonary fibrosis.MethodsPulmonary fibrosis was induced by bleomycin A5 in SD rats. Rats in drug group were killed after taking DHZC daily for 7 and 14 days. The lungs of all rats were harvested for histopathological study. The contents of Superoxide Dismutase(SOD)and lipid hydroperoxide (LPO) in bronchoalveolar lavage fluid were measured and compared. ResultsThe degree of the pulmonary fibrosis in bronchoalveolar lavage fluid was increased significantly in model group as compared with the normal group and content DHZC treated group(0. 05) . The activity of SOD in model group was lower than that in normal group on the 7th day and 14th day (0.05), and there was no significant difference between model group and DHZC treated group(Pgt;0. 05); the content of LPO in model group was higher than that in normal group on the 14th day (0.05), and the content of LPO in DHZC treat group was lower than that of model group(0.05).ConclusionDHZC can significantly decrease the content of LPO and alleviate the degree of alveolitis and pulmonary fibrosis induced by bleomycin A5 in SD rats. 　　Key words： Pulmonary fibrosis; Superoxide Dismutase(SOD); Lipid hydroperoxide (LPO); Dahuangzhechong (DHZC); Rat 肺间质纤维化是多种肺间质疾病发展到晚期的一种常见病理变化。虽然导致肺纤维化的原因不同,但其病理变化都从肺泡炎开始。一般认为肺纤维化改变是不可逆的, 西医治疗肺纤维化首选糖皮质激素，但只有部分如非特异性间质性肺炎（NSIP）和脱屑性间质性肺炎（DIP）等对激素反应较好，另一方面长期大剂量应用激素必将引起严重的代谢紊乱、免疫抑制和继发感染等不