大鼠肝移植急排模型的建立及相关免疫指标的检测_临床医学论文.docVIP

大鼠肝移植急排模型的建立及相关免疫指标的检测_临床医学论文 大鼠肝移植急排模型的建立及相关免疫指标的检测_临床医学论文 作者：彭勇　李敬东　肖江卫　刘海忠　刘作金　龚建平 【摘要】 目的：建立同种异体大鼠原位肝移植动物模型，观察急性排斥反应的发生发展以及相关免疫指标的变化过程。方法：Lewis大鼠与BN大鼠随机分为3组：正常对照组、同基因移植组及同种异基因移植组。观察大鼠存活率，移植组受体分别于术后3、5、7和10 d处死取标本，观察肝脏组织病理和超微结构变化，全自动生化分析法测定血清谷丙转氨酶（ALT）、总胆红素（total bilirubin，TBIL）和白蛋白（albumin，Alb）水平变化，ELISA检测血清IL-2含量。结果：在未用任何免疫抑制剂情况下，同基因组大鼠无急性排斥表现，14 d生存率为100%，肝组织发生轻度形态学改变，肝功能损害较轻，血清IL-2在正常范围。异基因组大鼠14 d内全部死亡，术后第7天肝组织病理改变出现典型急性排斥反应表现，肝脏功能损害较重，血清IL-2持续性升高，并在第7天达峰值。结论：Lewis大鼠与BN大鼠的组合方式，可以建立稳定的急排模型（急性排斥反应模型），在未用任何免疫抑制剂情况下，大鼠术后第7天急性排斥反应指标表现最为典型。 【关键词】 肝移植·急性排斥反应·白细胞介素2·大鼠 【ABSTRACT】 Objective: To establish the acute rejection model of orthotopic liver transplantation in rat (ROLT) and observe the basic pathophysiologic changes of acute rejection. Methods: The rats were randomly divided into three groups: control group, isotransplantation group (LEW-LEW), and allotransplantation group (LEW-BN). Recipients were sacrificed on 3, 5, 7, and 10 days postoperatively and liver tissues and blood samples were collected. Recipient survival rate, histopathological and ultrastructural characteristics were observed. ALT, TBIL, and Alb were measured with automatic biochemical analyser. IL-2 content in serum was assayed by ELISA. Results: There was no rejection in isotransplantation from Lewis to Lewis rat resulted and 14-day survival rate reached 100%. The IL-2 concentration in serum was at normal level. On the contrary, all recipients in allotransplantation group from LEW to BN rat died among 14 days postoperatively, and hepatic histological examination showed typical acute rejection on 7 days. Liver function was severely impaired, which was indicated by significant increase of ALT and TBIL levels and apparent decrease of Alb level. The IL-2 concentration in serum was continuously increased and reached its peak value on 7 days postoperatively. Conclusion: An acute rejection experimental model of liver transplantation in rat could be stably established using Lewis rat as donor and B