产番泻叶的泻下及抗骨质疏松作用研究_临床医学论文.docVIP

产番泻叶的泻下及抗骨质疏松作用研究_临床医学论文 产番泻叶的泻下及抗骨质疏松作用研究_临床医学论文 【摘要】 目的:探讨国产番泻叶的泻下及雌性激素样抗骨质疏松作用。方法:比较国产与进口商品番泻叶提取物对小鼠排便和肠蠕动作用的影响，观察其泻下通便的效果。并通过观察对去势雌性小鼠动情周期、子宫重、股骨重的变化和血钙、血磷、降钙素含量的影响，研究由国产番泻叶制备而得的番泻总苷提取物的雌性激素样抗骨质疏松作用。结果:两种商品番泻叶提取物与生理盐水对照组相比均能显著增加墨汁推进率、缩短首次排便时间，增加6 h排便量，两种商品之间无显著性差异。由国产番泻叶制备而得的番泻总苷提取物能缩短去势小鼠动情周期，有雌性激素样促进子宫增生和骨质钙化的作用。结论:国产番泻叶具有较好的泻下通便作用，并初步发现其具有雌性激素样抗骨质疏松作用。 【关键词】 番泻叶 泻下剂 骨质疏松 Abstract: Objective: To investigate the cathartic and anti-osteoporosis effect of native Folium sennae. Methods: Cathartic effect was assayed by measuring the action on defecation and intestinal peristalsis of mice treated with Folium sennae extracts. Inhibitory effect on osteoporosis similar to estrogen was investigated by observing estrous cycle, detecting weight of uterus and left femur, and value of plasma calcium, plasma phosphorus and calcitonin treated with purified total sennosides extracts. Results: Compared with saline control group, the group treated with Folium sennae extracts markedly enhanced peristaltic rate of intestine, shortened the time of first defecation and increased bulk of 6 hours stools. There was no significant difference between native and foreign Folium sennae on these aspects. The purified total sennosides extract shortened estrous cycle, improved weight of uterus and left femur, and decreased value of plasma calcium in ovariectomized mice. Conclusion: The results indicate that native Folium sennae possesses obvious cathartic effect, and the purified total sennosides extract from native Folium sennae exhibits inhibitory effect on osteoporosis similar to estrogen. Key words: Folium sennae; purgative agents; osteoporosis 番泻叶(Folium sennae)为中国药典2005年版一部收载的常用泻下导滞药之一，为豆科植物狭叶番泻(Cassia angustifolia Vahl)或光叶番泻(C. acutifolia Lelile)的干燥小叶[1]。 番泻叶味甘、苦，性寒，有泻热行滞、通便的功能。主要用于治疗热结便秘、积滞腹胀。近些年来发现番泻叶可治疗流行性出血热、急性胰腺炎及中毒性肠麻痹等[2，3]。本品长期以来依靠进口，主要进口国是印度。20世纪80年代以来，我国在海南、广西等省引种成功，但商品售价仅为进口的1/3～ 1/2。国产番泻叶外观、性状、显微特征与进口商品无明显差异，但其有效成分的多寡和活性如何还需要进一步研究。为此我们课题组从化学成分、活性成分的定性定量以及药理作用等多方面进行一系列的比