子宫内膜癌PTEN蛋白及P53蛋白的异常表达_临床医学论文.docVIP

子宫内膜癌PTEN蛋白及P53蛋白的异常表达_临床医学论文 子宫内膜癌PTEN蛋白及P53蛋白的异常表达_临床医学论文 【摘要】 目的 探讨子宫内膜癌组织抑癌基因PTEN及p53异常表达的意义。 方法 采用免疫组织化学SP法检测62例子宫内膜癌、16例子宫内膜增生过长和9例正常子宫内膜组织PTEN蛋白及P53蛋白的表达情况，表达率的差异采用卡方检验。 结果 子宫内膜癌、子宫内膜增生过长和正常子宫内膜组 的PTEN蛋白表达缺失率分别为64.52%、50%和11.11%，子宫内膜癌和子宫内膜增生过长组明显高于正常子宫内膜组（0.01）,但前两组间差异不显著；子宫内膜癌组、子宫内膜增生过长和正常子宫内膜组的P53蛋白阳性表达率分别为46.77%、50%和55.56%，三组间无显著差异（Pgt;0.05）；PTEN蛋白表达缺失和P53蛋白阳性表达与子宫内膜癌组织分化程度无相关关系(Pgt;0.05)；在子宫内膜癌组中，PTEN蛋白表达缺失组P53蛋白阳性表达率为37.50%，显著低于PTEN阳性表达组的63.64%(0.05)。 结论 PTEN蛋白在子宫内膜癌的发生中可能起着比P53蛋白更为重要的作用。 【关键词】 子宫内膜癌; 抑癌基因; PTEN; p53; 免疫组织化学 Abstract: Objective To study the significance of abnormal expression of tumor suppressor genes PTEN and p53 in endomatrial cancinoma. Methods Immunohistochemical method was used to detect the expression of PTEN and p53 in 62 cases of endomatrial carcinoma (EC),16 cases of endometrial hyperplasia (EH) and 9 cases of normal endometrium (NE). Results The negative expression rate of PTEN protein was 64.25% in EC and 50% in EH, significantly higher than that in NE (11.11%) (plt;0.01), but there was no significant difference between EC and EH. The positive expression rates of P53 protein in EC, EH and NE were 46.77 %, 50% and 55.56%, respectively, with no significant difference among them (Pgt;0.05). The deletion of protein expression and the positive expression of P53 protein were not significantly related with the differentiation of endomatrial carcinoma (Pgt;0.05). The positive expression rate of P53 protein in the PTEN negative expression and positive expression groups were 37.50% and 63.64%, respectively, and the former was significantly lower than the later (0.05). Conclusion PTEN may play a more important role than P53 protein in endomatrial carcinogenesis. Key words: endometrial carcinoma; tumor suppressor genes; PTEN; p53; immunohistochemistry 子宫内膜癌是女性生殖系统常见的恶性肿瘤之一，占女性生殖系统恶性肿瘤总数的20%～30%，且近年来发病率逐年上升，但其发病机制尚不完全清晰［1,2］。一般认为肿瘤的发生与抑癌基因突变累积有关。由于抑癌基因的突变，造成了其编码蛋白抑制细胞恶性增殖的功能降低，细胞不断增殖，累积到一定程度可能导致细胞恶性转化，肿瘤发生。PTEN（phophatase and tensinhomo