妊娠高血压综合征患者血清中抗氧化物与胎盘Bax、Bcl-2关系的探讨_临床医学论文.docVIP

妊娠高血压综合征患者血清中抗氧化物与胎盘Bax、Bcl-2关系的探讨_临床医学论文 妊娠高血压综合征患者血清中抗氧化物与胎盘Bax、Bcl-2关系的探讨_临床医学论文 【摘要】 目的 探讨妊娠高血压综合征(PIH)患者外周血清中抗氧化物和胎盘组织细胞Bcl-2、Bax蛋白表达水平的关系。方法 检测68例PIH病人(PIH组)和25例正常孕妇(NLP 组)血清中GSH-px、MDA 、SOD 活性,并用免疫组织化学SP法检测胎盘组织Bax、Bcl-2蛋白并进行比较。结果 PIH中、重度组血清GSH-px/ MDA、SOD/ MDA、Bcl-2/Bax 比率明显低于NLP组(P＜0.05);PIH中、重度组胎盘组织细胞凋亡指数、Bax高于NLP组(P＜0.01);各组P53蛋白均未见阳性表达。血清GSH-px/MDA、SOD/MDA与凋亡指数、Bcl-2/Bax呈正相关(P＜ 0.01)。结论 PIH的发生与机体氧化和抗氧化平衡失调及与其引起的胎盘组织细胞凋亡增加有关。 【关键词】 妊娠高血压综合征 氧化和抗氧化平衡 Bax蛋白 P53蛋白 　　Relationship Between Serum Antioxidants and the Expression of Bcl2、Bax Apoptosis Protein of Placental Tissues with Pregnancy 　　1.School of Basic Medicine, Ningxia Medical College, Yinchuan 750004; 2.City Health Care Hospital for Women and Children,Yinchuan 750001 　　Abstract: Objective To explore the relationship between superoxide dismutase (SOD), glutathione peroxidase (GSH、Bax apoptosis protein of placental tissues with pregnancyyndrome (PIH). Methods The activity of SOD, GSHpx and content of MDA in the serum of peripheral blood of nonpregnant women, normal pregnant women and PIH women were measured and the expression of Bcl2, Bax were assessed by immunolistochemical detection. Results The ratio of GSHpx/MDA、SOD/MDA、Bcl＜0.05),apoptosis index (AI)and the expression of Bax in normal pregnant women were significantly higher than those in nonpregnant women(P＜0.05)； there were significantly positive correlation in the ratio between GSH、SOD/MDAand Bcl、apoptosis index (0.05). Conclusion The mechanism of PIH is associated with the disequilibrium of oxidation,anti oxidation and apoptosis overexpression of placental tissues induced by it. 　　Key words: pregnancy；disequilibrium of oxidation and antioxidation；Bax； P53 　　妊娠高血压综合征(pregnancy-induced hypertension，PIH)是影响母儿围生期发生率和死亡率的重要因素之一,其病因及发病机制至今尚未完全阐明。大量实验证明PIH患者胎盘组织凋亡增加［1］，细胞凋亡由一系列基因调控,其中促凋基因Bax和抑凋基因Bcl-2被认为是非常重要的成员,Bcl-2/Bax的比率决定了细胞对刺激的敏感性。为此,本实验检测PTH 胎盘组织细胞的凋亡、凋亡相关基因Bax、Bcl-2蛋白,以及PIH患者血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)活性及丙二醛(MDA)含量的变化,旨在探讨SOD、GSH-p