姜黄素与依那普利对大鼠肾小管上皮细胞转分化影响的对比研究_临床医学论文.docVIP

姜黄素与依那普利对大鼠肾小管上皮细胞转分化影响的对比研究_临床医学论文 姜黄素与依那普利对大鼠肾小管上皮细胞转分化影响的对比研究_临床医学论文 作者：赵爱青,张晓明,侯恒,李荣山,马存根 【摘要】 目的 探讨姜黄素对单侧输尿管梗阻(UUO)大鼠肾小管上皮细胞转分化的作用。方法 采用UUO致肾间质纤维化大鼠模型。将大鼠随机分为假手术组、模型组、姜黄素组、依那普利组。从造模后第2天起,姜黄素组、依那普利组分别给予100 mg/(kg·d)姜黄素、10 mg/(kg·d)依那普利腹腔注射。术后第4周,处死各组大鼠,用HE、Masson染色评定肾小管间质纤维化程度；用免疫组化方法检测肾组织内转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)、波形蛋白(Vimentin)的表达部位及蛋白表达水平。结果 与假手术组相比,模型组大鼠血清尿素氮水平显著增加(0.01),肾间质纤维组织相对面积显著扩大(0.01),肾组织内TGF-β1、α-SMA、Vimentin表达均显著上调(0.01)。姜黄素或依那普利干预后,上述上调指标都被显著抑制(0.05或0.01)。两种药物之间比较,作用无显著性差异(Pgt;0.05)。结论 姜黄素及依那普利可能通过抑制UUO大鼠肾小管上皮细胞转分化而减轻肾间质纤维化。 【关键词】 姜黄素；依那普利；α-平滑肌肌动蛋白；波形蛋白；肾小管上皮细胞转分化；大鼠 Abstract：Objective To compare the effects of curcumin and enalapril on transdifferentiation of renal tubular epithelial cells in unilateral ureteral obstruction (UUO) rats. Method UUO rat model was used. Male wistar rats were randomly divided into four groups with 8 rats each：shame-operated group (0.9% saline solution), model group (dimethyl sulphoxide), curcumin treatment group [curcumin 100 mg/(kg·d)], and enalapril treatment group [enalapril 10 mg/(kg·d)]. Medicines were given intraperitoneally daily two days after the operation. All rats were killed 4w after surgery. The degree of tubulointerstitial fibrosis was scored by HE and Masson staining, and the sites and levels of protein expression of TGF-β1、α-SMA and vimentin were detected by immunohistochemistry staining. Result Compared with shame-operated group, the serum urea nitrogen level was significantly increased (0.01), and the relative area of interstitial fibrosis was also significantly enlarged in the model group (0.01), the expression of TGF-β1、α-SMA and Vimentin protein was significantly up-regulated in their kidney tissue (0.01). After intervention with curcumin or enalapril, the up-regulation of the above-mentioned parameters were all significantly inhibited (0.05 or 0.01). There was no significant difference of intervention effects between curcumin and enalapril (Pgt;0.05). Conclusion The pharmacological eff