妊娠组织中转化生长因子及其受体与早期复发性自然流产相关性研究_临床医学论文.docVIP

妊娠组织中转化生长因子及其受体与早期复发性自然流产相关性研究_临床医学论文 妊娠组织中转化生长因子及其受体与早期复发性自然流产相关性研究_临床医学论文 作者：韦枝红 宁克勤 龙淑芳 徐小平 【摘要】 目的：了解转化生长因子β1（TGF -β1）及其受体（TβR、Ⅰ、Ⅱ）在绒毛及蜕膜组织中的表达情况，初步探讨（TGF -β1）及（TβR）与早期复发性自然流产（RSA）的关系。方法：应用半定量逆转录 - 聚合酶链反应（RT-PCR）技术，检测 38 例 RSA 患者和 35 例正常早孕妇女的绒毛及蜕膜组织中 TGF -β1 mRNA，TβR（Ⅰ、Ⅱ型）mRNA 的表达情况。结果：TGF -β1 及 TβR、Ⅰ、Ⅱ型在正常早孕及 RSA 绒毛、蜕膜组织中均有表达，TGF -β1 在 RSA 组绒毛及蜕膜组织中表达水平为（0.18±0.02）、（0.12±0.01），对照组为（0.98±0.03）、（0.72±0.02）；TβR -Ⅰ在 RSA 组绒毛 及蜕膜组织中表达水平为（0.15±0.03）、（0.11±0.03），对照组为（0.71±0.01）、（0.73±0.01）；TβR -Ⅱ在 RSA 组 绒毛及蜕膜组织中表达水平为（0.16±0.01）、（0.09±0.01），对照组为（0.68±0.02）、（0.86±0.02）；上述指标 RSA组与对照组比较差异均有统计学意义（P lt; 0.01）。结论：TGF -β1 及 TβR 在绒毛及蜕膜组织中表达下降可能是早期复发性自然流产的重要原因之一。 【关键词】 转化生长因子β1；受体；复发性自然流产；滋养细胞；蜕膜 〔Abstract〕 Objective To understand the expressions of transforming growth factor β1 (TGF -β1) and its receptors (TβR) in villi and decidua during early pregnancy and their effects on early recurrent spontaneous abortion. Methods Expressions of TGF -β1 mRNA and its receptors were detemined in villi and deciduas by semi-quantitative reverse transcriptional-polymerase chain reaction from 38 cases of spontaneous abortion women and 35 cases of normal early pregnancy women (control group). Results TGF -β1 and its receptors had expressioned in decidua cells, villis glands and interstitial cytoplasm in all two groups. The expressions of TGF -β1 in the villi and decidua of spontaneous abortion and control group were (0.18±0.02), (0.98±0.03) and (0.12±0.01), (0.72±0.02) respectively. The intensity of TβR -Ⅰ in the villi and decidua of spontaneous abortion and control groups were (0.15±0.03), (0.71±0.01) and (0.11±0.03), (0.73±0.01) respectively. And the expressions of Tβ1R -Ⅱ in the villi and decidua of spontaneous aboetion and control groups were (0.16±0.01), (0.68±0.02) and (0.09±0.01), (0.86±0.02) respectively. The differences between the control group and abortion group were all significant (P lt; 0.01). Conclusion The expressions of TGF -β1 and its receptors in the early pregnancy tissues