体外循环前后不同组织细胞因子的差异表达_临床医学论文.docVIP

体外循环前后不同组织细胞因子的差异表达_临床医学论文 体外循环前后不同组织细胞因子的差异表达_临床医学论文 作者：齐弘炜，吴明营，韩欣刚，陈楠，张吉涛，赵凤华，孙婷婷，朱朗标 【摘要】 目的 应用基因芯片技术探讨外周血单个核细胞(PBMC)及心房组织细胞因子的差异表达基因，探讨不同组织对体外循环(CPB)的反应。方法 CPB开始、CPB结束即刻抽取患者动脉血，密度梯度离心法分离PBMC;取CPB开始及CPB结束即刻的右房组织。用BD AtlasTM cDNA Expression Arrays表达谱基因芯片对比CPB前及CPB后二者间细胞因子的差异表达。结果 CPB前PBMC及心房间细胞因子基因表达差异明显者有IL-6、IL-13、Wnt5a、促肾上腺皮质激素释放激素Ⅰ型受体、卵泡刺激素受体和盘状结构域受体2。CPB后二者的基因表达谱均发生变化，差异明显者变为IL-6、IL-13、α-干扰素诱导蛋白(IFI616)、钙结合蛋白S100A9(钙颗粒素B)、胎盘生长因子和Wnt5a。结论 CPB前PBMC与心房组织的细胞因子基因表达谱不同，CPB刺激后基因表达谱发生变化，但变化不一致，使差异表达基因发生变化。 【关键词】 细胞因子;基因表达;基因芯片;外周血单个核细胞;心肌;体外循环 　　Abstract: OBJECTIVE To determine the differentially expressed genes of cytokines between peripheral blood mononuclear cell (PBMC) and right atrium using gene chip technique. To explore the reactions to cardiopulmonary bypass(CPB) in the different tissues.METHODS The patients oxygenated bloods were drawn immediately before onset and termination of CPB. PBMC were obtained from heparinised blood by Ficoll gradient centrifugation. Right atrium were collected just before onset and termination of CPB. The differential expression between PBMC and atrium was compared using BD AtlasTM cDNA Expression Arrays. RESULTS Between PBMC and right atrium before CPB, the differentially expressed genes were interleukin 6, interleukin 13, Wnt5a, corticotropin releasing hormone receptor 1 (CRH1R), follicle stimulating hormone receptor (FSH-R) and discoidin domain receptor family, member 2 (discoidin domain receptor, DDR2). Both of the gene expression profiles changed after CPB. The differentially expressed genes between PBMC and right atrium were interleukin 6, interleukin 13, interferon alpha-inducible protein (clone IFI-6-16), S100 calcium binding protein A9 (calgranulin B), placental growth factor and Wnt5a. CONCLUSION The gene expression profiles in cytokines of PBMC and right atrium were different before CPB. The gene expression profiles changed in different manners after CPB. So, the differentially expressed genes changed. 　　Key words： Cyto