壳聚糖亲与膜脱除蛋白质溶液中内毒素的研究.pdfVIP

B066 壳聚糖亲和膜脱除蛋白质溶液中内毒素的研究 孙海翔 张林 柴红 陈欢林* （浙江大学材化学院生物工程研究所，杭州 310027 ） 摘 要 对聚偏氟乙烯（PVDF）中空纤维膜进行表面亲水性处理后，键合壳聚糖配基，制成壳聚糖亲和膜。 FTIR 分析表明，改性后 PVDF 膜表面的氨基、羟基明显增加， 氟含量降低。实验测试发现，在离子强度 为 0.2mol/L，pH为 5～8，流速为 1.0 mL/min 的条件下，水溶液中内毒素的去除率为91.2 ％；亲和膜对内 毒素的饱和吸附量为 21.421Eu/mg 膜，解离常数为 0.5001Eu/mL，符合 Langmuir 等温吸附模型。壳聚糖 亲和膜用于去除BSA溶液中的内毒素时，在溶液 pH 为 5，离子强度为 0.2mol/L 条件下，其对内毒素去除 率达 88.6 ％，BSA回收率为93.4 ％；用于溶菌酶溶液中内毒素的去除时，在pH为11，离子强度为0.2mol/L 条件下，内毒素的去除率仅为 76.4 ％，溶菌酶回收率为92.3 ％。 关键词 壳聚糖; 聚偏氟乙烯 (PVDF); 内毒素去除; 亲和吸附. STUDY OF ENDOTOXIN REMOVAL FROM PROTEIN SOLUTION BY CHITOSAN AFFINITY MEMBRANE SUN Haixiang, ZHANG Lin, CHAI Hong and CHEN Huanlin （Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027,China ） Abstract A chitosan affinity membrane was prepared with hydrophile-modifided poly(vinylidene fluoride) (PVDF) hollow fiber membrane used as the matrix and chitosan as ligand immobilized on the activated membrane. FTIR analysis indicated that after modification the contents of NH2- and OH- on the membrane surface increased and the content of fluoride decreased. The effects of operating parameters, such as pH, ionic strength and flow rate, on the removal of endotoxin were also investigated. The results showed that the equilibrium adsorption capacity (qm) of endotoxin and the desorption constant (Kd) of the affinity membrane were 21.421Eu/mg membrane and 0.5001Eu/mL, respectively, at the conditions of pH 7, ionic strength 0.2 mol/L, which appeared to follow a typical Langmuir isotherm. At pH 5.0, ionic strength 0.2 mol/L, the removal rate of endotoxin from BSA solution with the chitosan affinity membrane was up to 88.6%, and the recovery of BSA was 93.4%, while at pH 11.0, ionic strength 0.2 mol/L, the