载牛血清白蛋白微胶囊的制备与其体外缓释的研究.pdfVIP

M049 载牛血清白蛋白微胶囊的制备及其体外缓释的研究 张杰 张新宇 郭妮妮 张代佳 修志龙 (大连理工大学环境与生命学院生物科学与工程系，大连116024) 摘 要 采用喷雾法制备了载牛血清白蛋白（BSA ）的海藻酸钙微胶囊。通过比较不同条件下微 胶囊的平均粒径和载药率，优化了喷雾法的制备条件和溶液中微胶囊的分离条件。结果表明：控 3 制 CaCl2 溶液浓度为 1.1% （w/v ）、8#针头、气速为 1.0m /h 、蠕动泵速度为 15rpm，所制备的微 胶囊粒径最小（平均粒径为 50-60 μm ），且包埋率可达 50% 以上。最后，比较了在不同释放液中， 喷雾法制得的载 BSA 的微胶囊的体外释放曲线。 关键词 海藻酸盐微胶囊 牛血清白蛋白 喷雾造粒法 Preparation of Alginate Microcapsules Loading BSA by Spray and Release of BSA In Vitro Jie Zhang, Xinyu Zhang, Nini Guo, Daijia Zhang and Zhilong Xiu (Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024) Abstract The alginate microcapsules loading bovine serum albumin (BSA) as a model drug were prepared by spraying. The conditions of preparation were optimized by comparing the average diameters of particles and the loading capacity of BSA under different conditions. The results indicated that the microcapsules with average diameter of 50-60 μm were prepared by mean of 8# type of syringe needle at a concentration of CaCl2 of 1.1% (w/v), a gas velocity of 3 1.0m /h, and a rate of peristaltic pump of 15 rpm. The size distribution of particles was similar to Gauss distribution and the loading ratio of BSA was higher than 50%. The release of BSA was also investigated under different media. Keywords Alginate microcapsule, BSA , Spraying 作者简介：张杰（1979-），女，博士生；修志龙，（1965-），男，博士，教授，博士生导师， 通讯联系人，Tel/Fax: 0411E-mail:zlxiu@mail.dlptt.ln.cn; 地址：大连市甘井子区 凌工路 2 号（116024）。 引 言 微胶囊控释系统是近几十年来发展起来的一种新型控释给药体系。它是将生化类药物[1,2]、 酯溶性药物[3] [4] 以及中药的有效成分 等包覆