MTBE高效降解菌的分离鉴定及降解特性研究.pdfVIP

农业环境科学学报200726(6)：2080—2084 Joumal Scicnee ofAgro-Environment MTBE高效降解菌的分离鉴定及降解特性研究 郑艳梅，李鑫钢，黄国强 (天津大学化工学院精馏技术国家工程研究中心，天津300072) 摘要：通过对取白天津大港油田的土著菌进行培养驯化．分离筛选得到适宜MTBE(甲基叔丁基醚)降解的优势菌种，经生理生化 实验结果、菌落特征、菌体形态以及16SrDNA测序结果，鉴定出该菌种为蜡状芽孢杆菌Bacilluscerr3[1$，利用问歇反应实验确定了 MTBE降解的适宜条件：温度为25℃，pH值为8．5，接种量30％。 关键词：MTBE；生物降解：菌种鉴定 中图分类号：X172文献标识码：A 文章编号：1672—2043(2007)0“2080—05 MTBE Isolation．IdentificationandCharacteristicsofA Strainfor Degradation ZHENG Yan-mei，L1 Guo-qlaug Xin—gang，HUANG ofChemical and CenterforDistillation (School Enginee6ngTechnalogy，NationalSnglneefingResearch Technology，Tianjin300072，Chi na、 ofitstasteandedourthresholdand texici— Abstract：MethyltertiarybutyletheHMTBE)，alloxygen-hearinghydroearhen，because suspected fits hasbeen and80meformoftreatmentismandatedin mixedcultureonestrainidentifiedBacillus CaSeS．Through ty，MTBE regulated many thatcould and fromthe Od i— c￡Felt$，which MTBE，Ⅷisolated Field，Ti删in．The rapidlydegrade byenriching，screeningculturing Dagang for dentifiedresultswerebasedonthe and16SrDNA eondifitmsMTBE sequence optimized morphology，biochemicalproperties analysis．The aerobic weredeterminedbvintermittentreaction was value theinee— bledegradation experiments∞followed：temperature25℃，pH 8．5，and ulationconcentration thedissolvedconcentration isshownthatMTBECallnatbe intlera