部分古桂遗传多样性的ISSR的分析初稿.docVIP

古桂遗传多样性与亲缘关系的ISSR分析 段一凡，王贤荣(，梁丽丽，李雪霞，向其柏，赵正萍 (南京林业大学森林资源与环境学院，南京，210037) 摘要：利用ISSR分子标记，以云南木犀(Osmanthus yunnanensis (Franchet) P.S.Green)和柊树（Osmanthus heterophyllus （G.Don.Shannon多样性信息指数分别为1.8851、1.4106、0.2415和0.3688；聚类分析结果表明对照种与桂花遗传距离较远，最早分开；古桂花具有很强的地理相关性，来自同一来源地或具有相似颜色的个体往往聚在一起；四季桂与其他秋桂类个体遗传距离较远。 关键词：古桂；ISSR；遗传多样性；聚类分析 中图分类号：S792.95；TQ920.1 Analysis of genetic diversity and relationships among ancient sweet osmanthus trees using ISSR markers DUAN Yi-fan,WANG Xian-rong ,LIANG Li-li,LI Xue-xia,QIANG Qi-bai,ZHAO Zheng-pin （College of Forest Resources and Environment, Nanjing Foresty University,Nanjing,210037) Abstract: Interspecific relationships of 45 ancient sweet osmanthus trees from 6 provinces and 2 wild species of sweet osmanthus were evaluated by using ISSR (inter simple sequence repeat) marker with Osmanthus yunnanensis (Franchet) P.S. Green and Osmanthus fordii Hemsl as contrast species. The results showed that ancient sweet osmanthus trees had abundant genetic diversity. Fourteen ISSR primers produced a total of 148 bands, of which 131 were polymorphic. The percentage of polymorphic bands was 88.51%. The mean observed number of alleles, effective number of alleles, Nei’s genetic distance and Shannon index were 1.8851, 1.4106, 0.2415 and 0.3688 respectively. The cluster analysis showed that the genetic distance between contrast species and sweet osmanthus was far. Ancient sweet osmanthus trees were closely correlated with geographical locations, and trees from the same place or in similar color often clustered together. The genetic distance between Asiaticus Group and others was far. Key words: ancient sweet osmanthus trees, ISSR, genetic diversity, cluster analysis 桂花Osmanthus fragrans (Thunb.) Lour.属木犀科(Oleaceae)木犀属(Osmanthus Lour.)植物，，2500余年的栽培历史[1]。桂花是长寿树种，在我国很多地方都有树龄超过100年的古桂存在[2]。中华古桂是中国古老文明的象征，是活的文物标本，也是研究古气候、古环境、古树种质资源以及桂花新品种培育的重要材料，同时还是重要的旅游资源[3]。桂花遗传多样性研究已多有报道，但研究材料几乎都为新近培育的栽培品种，针对古桂的遗传多样性研究还十分有限，其分子层面研究还未见报道[2-15]。 ISSR（inter simple sequence repea