毕业论文-硒化乳酸菌胞外多糖理化性质及其抗氧化活性研究.doc

题目 硒化乳酸菌胞外多糖理化性质及其抗氧化活性研究 作者姓名 指导教师 二级学院 西安培华学院 专 业 药学 学 号 9B093122  硒化乳酸菌胞外多糖理化性质及其抗氧化活性研究 摘 要 目的：本文对制备的硒化乳酸菌胞外多糖进行了理化性质及其抗氧化活性的研究。 方法：通过理化性质实验得出，它是一种溶于水，不溶于乙醇，丙酮，氯仿等有机溶剂的不含淀粉多糖的多糖；通过硒化与未经硒化乳酸菌胞外多糖清除超氧自由基，羟自由基的能力以及他们的总抗氧化能力实验。 结果：经硒化的乳酸菌胞外多糖在多糖浓度为8mg/mL时对超氧阴离子自由基清除率为61.25%，相比硒化前的多糖在同样浓度下对超氧阴离子自由基的清除率41.74%提高了19.51%；而在多糖浓度为8mg/mL时对羟自由基的清除率从54.94%提高到74.18%，提高了19.24%；在多糖浓度为10mg/mL时，硒多糖的总抗氧化能力为9.7（U/L），未经硒化的总抗氧化能力7.4（U/L），提高了2.3（U/L）。 结论：说明硒化乳酸菌胞外多糖比未硒化乳酸菌胞外多糖的抗氧化活性更强。 关键词：乳酸菌；硒多糖；抗氧化活性 The research on Antioxidant activity to Selenium Exopolysaccharides of lactic acid bacteria Abstract Purpose:A research of physical and chemical properties and antioxidant activity to selenium exopolysaccharides of lactic acid bacteria in this paper. Methods：The physical and chemical properties experiments showed that selenium exopolysaccharides of lactic acid bacteria is a polysaccharide that soluble in water but insoluble in organic solvents like ethanol, acetone, chloroform,etc, and is non-starch polysaccharides; The scavenging of superoxide radical and hydroxyl radical and total antioxidant capacity to selenium andnon-seleniumexopo-lysaccharides of lactic acid bacteria was researched. Results:The results showed that the superoxide anion radical scavenging rate was 61.25% in the selenium exopolysaccharides of lactic acid bacteria concentration 8mg/mL, compared to the scavenging rate of non-selenium exopolysaccharides of lactic acid bacteria 41.74% increase 19.24% from 54.94% to 74.18%, when the concentration of 10mg/mL in polysaccharide, the total antioxidant capacity of polysaccharide increased 2.3（U/L） from 7.4（U/L） to 9.7（U/L）. Conclusion: Description Selenium exopolysaccharides of lactic acid bacteria than lactic acid bacteria without EPS selenium antioxidant activity stronger. Keywords：Lactic acid bacteria; Selenium Polysaccharide; Antioxidant activity 目 录 引 言 1 1．材料 2 1.1 材料 2 1.2仪器与设备 2 1.3试剂 3 2．方法