小麦成熟胚再生体系影响因素的研究[J].pdfVIP

, JournalofAnhuiAgr.i Sci. 2010, 38( 15): 8276- 8278 李菲菲 卢瑶 贺杰, 曹银萍,胡海燕, 赵俊杰,周 岩 (, 453003) [目的]研究小麦离体培养的影响因素,探索小麦成熟胚 伤组织诱导继代分化和植株再生的有效方法, 建立 一套成熟的植株 再生体系,为进一步转基因研究奠定基础[方法]采用河南的 5 个不同小麦品种, 经小麦种子消毒,无菌取胚,然后接入诱导培养基进 行 伤组织诱导, 伤组织再经继代培养分化培养以至小麦植株再生通过对它们浸种时间和成熟胚离体培养过程的观察,研究了不 同预处理时间和植物激素, 如 2, 4DABAKTIAA和 NAA, 对小麦成熟胚 伤组织在诱导继代分化和植株再生方面的 一些影响 [结果] 15 h的浸种时间效果好; 4. 0mg/L 2, 4D有利于 伤组织形成;在继代培养基中添加0. 3mg/LABA有利于小麦致密 伤组织的 诱导及再生能力的保持; KT和 6BA 对提高芽的分化有显著作用; 附加浓度 1. 5mg/L NAA和0. 2mg/L AI A的 1/2MS培养基可有效促 进生根[结论]浸种时间基因型对小麦 伤组织的继代分化和再生有很大的影响, 添加一定的外源激素有利于提高其植株的再生 频率 小麦;成熟胚; 伤组织;植株再生 S512. 1 A 0517- 6611(2010) 15- 08276- 03 R esearch on the Fa ctor A ffecting the R egenera tion System from WheatM a ture Em bryo Culturing HE J ie et al ( enan Science andTechnology College, Xinxiang, enan 453003) A bstra ct [Objective] The foundation for thefurther research on transgenic plantwas laid through the establishmentof amature plantregenera tion system based on the study thefactors influencingwheatculture in vitro for effectiveway of the callus induction and subculture, the differentia tion and the plant regeneration ofwheatmature embryo. [Method] The sterile embryos of five differentwheatvarieties from enanprovincew ere cut and cultured for callus inductionafter thewheat seedsweredisinfected, and the calliwere subculturedanddifferentially cultured forplantre generation. The mi pactof the different preprocessing tmi esand plant hormones, such as2, 4D, ABA, KT, IAA andNAA, on the induction, the subculture, the differentiation and the regeneration ofwheat calluswas studied through the investigation in the soaking them and the process of mature embryo culture invitro. [Results] The result from the soaking tmi e of 15hourswas good; the 2, 4D of4. 0mg/Lwas conducive to callus induction; theABA 0. 3mg/L added in subculturemedium was in favor of the formation of compact callus from wheat embryo culture