毕业设计（论文）-细野荞和苦荞清除活性氧作用研究.docVIP

毕 业 论 文 题 目：细野菌和苦荞清除活性氧 作用研究 学 号： 08110902033 姓 名： 教 学 院： 地理与生命科学学院 专业班级： 生物科学2009级 指导教师： ） 细野荞和苦荞清除活性氧作用研究 作者姓名： 专业班级：生物科学2009级 学号：08110901033 指导教师： 关键词：细野荞；苦荞；活性氧；·OH ；·O2- ；H2O2 Scavening of eactive Oxygen Species by the Extraction of F. gracilipes(Hemsl) Dammer ex Dielst and F. tartaricum(L.)Gaertn Candidate: Zhao fei-fei Major: Biology science Student No.:08110901033 Advisor: Zhang yizhong Abstract: This experiment use the method of NBT photoreduction reduction, the system of fenton and Ammonium molybdate spectrophotometry to study the cleaning effect of extracted liquor from F.gracilipes(Hemsl) Dammer ex Dielst and F.tartaricum(L.)Gaertn (leaves, stem, root) on ·OH,·O2- and H2O2. The results showed that the cleaning effect of the stem from F.gracilipes(Hemsl) Dammer ex Dielst was more respectively significant than the leaves and root on ·OH and H2O2; the cleaning effect of the leaves was more respectively significant than the root and stem on ?O2-, meanwhile the stem was more significant than the root, but the significant level was not reached. The cleaning effect of the stem and leaves from F.tartaricum(L.)Gaertn more respectively significant than the root and stem on ·OH and ?O2-, the cleaning effect of the leaves was more respectively significant than the root and stem, but it was not reached the significance level, and the cleaning rate of the root and stem was not significant in difference on ?O2-. Key words: F.gracilipes(Hemsl) Dammer ex Dielst; F.tartaricum(L.)Gaertn; Reactive oxygen species; ·OH; H2O2; ·O2–. 目 录 摘 要 i Abstract. ii 引言 1 1 材料和方法 2 1.1 供试材料 2 1.2仪器与试剂 2 1.3试验方法 2 1.3.1 提取液对·OH的清除测定方法 2 1.3.2 提取液对·O2-的清除测定方法 3 1.3.3 双氧水H2O2的研究 4 1.3.4 计算公式 4 2 数据处理 4 3 结果与分析 5 3.1细野荞各器官提取液对·OH的清除作用 5 3.2细野荞各器官提取液对·O2-的清除作用 5 3.3细野荞各器官提取液对H2O2的清除作用 6 3.4 苦荞各器官提取液对·OH的清除作用 7 3.5 苦荞各器官提取液对·O2-的清除作用 8 3.6 苦荞各器官提取液对H2O2的清除作用 8 4 讨论 9 参考文献 11 致 谢 13 引言 荞麦（Buckwheat）又名三角麦、乌麦，属蓼科（Polygonac