冰片和复方制剂中冰片的药物动力学比较研究.pdfVIP

第十一届中南地区实验动物科技交流会论文集 冰片及复方制剂中冰片的药物动力学比较研究 苏小茹 (广州中医药大学，广东 广州 510405) 【摘要】1. 以 GC—MS 联用技术测定大鼠灌服复方丹参滴丸后其血清中的冰片含量。 冰片浓度在 0.00505—40.1μg/mI 范围内线性关系良好。血中回收率达 72.82—86.77 ％ ，最 低检测浓度为 1ng/mL。本方法以萘为内标，SIM 方式检测，定量准确、干扰少， 日内及 日间的RSD 小于 2.76 ％和 1.93％ ：结果表明本方法可应用于复方丹参滴丸所含冰片的药代 动力学等的进一步的研究。 【关键词】复方丹参滴丸/化学;冰片／分析；GC 一 MS 【摘要】2. 比较研究冰片和复方丹参滴丸中的冰片在大鼠体内的药物动力学。用建立的 方法测定大鼠单剂量灌服冰片、含等量冰片的复方丹参滴丸后一系列时间点冰片的血中浓 度。应用PK Solu 2.0 药物动力学软件(USA)处理药--时曲线数据，求得冰片的药物动力学参 数。结果等剂量的冰片和复方丹参滴丸中的冰片的药物动力学参数有显著差异，单味冰片的 -1 -1 -1 吸收相，分布相及清除相的速率常数分别为 2.630 h ，1.788 h 及 0.273 h ，远大于复方丹 参滴丸中等量冰片相应的速率常数(1.635 h-1 ，0.784 h-1和0.116 h-1) 。单味冰片的达峰时间(0.5 h)早于复方丹参滴丸(0.75 h)，但峰浓度 16.8μg ／mL约为复方丹参滴丸的 1/2(31.02μg ／mL) ， 药后 18 h的浓度(0.043μg ／mL)约为复方丹参滴丸的l/10 。因此中药(复方)药物动力学研究中 应注意其他成分(组成)对所研究(观察)成分药物动力学的影响。 【关键词】冰片；复方丹参滴丸；GS—MS ；药物动力学 【Abstract 】1. A specific and sensitive GC—MS method for assaying Borneol in the serum of rats medicated orally with compound Danshen dropping pill was developed. Naphthalene was used as the internal standard, the dectection was performed by SIM. The calibration curve was linear in the range from 0.00505 to 40.1μg/mL, the detectable limit of this method was 1ng/mL. The extraction recovery of Borneol was 72.82%--86.77%.The RSD of within — day — to – day was less than 2.76% and 1.93% respectively. The results showed that this method was simple, sensitive, accurate and good reproducibility, and can be used in further pharmacokinetic study of Borneol in danshen Dropping pill. 【Keyword 】 Conpound Danshen dropping pill/chemistry; Borneol/analysis; GC—MS. 【Abstract 】2. Objective To conpare the pharmacokinetics of borneolum syntheticum(BS) and borneol in compound Danshen dropping pills in rats. Method Rats were administrated orally