产金属_内酰胺酶铜绿假单胞菌的基因检测及耐药性研究_彭少华.pdfVIP

2004, 20 ( 5) Chinese ournal of Zoonoses 4 13 : 1002- 2694( 2004) 05- 0413- 04 产金属B- 内酰胺酶铜绿假单胞菌的基因检测 及耐药性研究* 彭少华, 朱 琴, 顾 剑, 金正江, 李从荣 : 研究本地区铜绿假单胞菌临床分离株中产金属B- 内酰胺酶流行株的主要基因 和耐药性 用纸 片扩散法进行抗生素协同敏感试验, 检测产金属B- 内酰胺酶的铜绿假单胞菌, 用琼脂稀释法检测其最低抑菌浓度( M I C) , 分 别用金属酶IMP - 1, IMP- 2, V IM - 1 和V IM - 2 引物进行聚合酶链反应( PCR) 并对扩增产物进行序列分析 在 82 株耐亚胺培南铜绿假单胞菌中, 抗生素协同敏感试验阳性7 株, PCR 检测金属B- 内酰胺酶阳性株也为这7 株, 且基因 均为 VIM - 2 产酶株对亚胺培南头孢噻肟头孢他啶哌拉西林/ 他唑巴坦等常用抗铜绿假单胞菌B- 内酰胺类抗生素不敏 感, 对氨曲南较为敏感 本地区铜绿假单胞菌所产金属B- 内酰胺酶为V IM - 2 , 且产酶株耐药性强, 应注意对其进 行临床检测和监控 :B- 内酰胺酶; 铜绿假单胞菌; 基因 + : R378199 1 : A Genotypesandresistanceof metallo-beta-lactamase-producing Pseudomonas aeruginosa PENG Shao-hua, ZHU Qin, GU ian, IN Zheng-jiang ( R enm in H osp ital , W uhan Univ ersity , Wuhan, H ubei 430060, China) ABSTRACT:T o investigate the genotypical char acterist ics and sensitivity of metallo-beta-lactamases in clinical isolates of Pse u- domonas aer ug inosa1 The clin ical isolates of metallo- beta-lactamase-p r od ucing Pseudomonas aer ug inosa were confirmed by synerg ic sensitivity tests and the M IC w er e determined by ag ar dilution method. PCR were performed using primers specific for blaIMP- 1, blaIMP-2, blaVIM- 1 and blaV IM-2 respectively , and the P CR products were purified and sequenced1 If was found that metallo-beta-lacta- mase-producing strains w ere unsensitive to ceftazidime, cefotax ime, imipenem and piperacillin/ tazobactam1 PCR exper iment g ave blaV- I M-positive r esults for all the strains, and the P CR pr oducts w ere identified w ith V IM-2 by DNA sequencing1 If concludes that VI M- 2 is t he genoty pe of metallo-beta-lactamases in clinical isolates of Pseudomonas