利用基础导入系分析粳稻基因的遗传效应.pdfVIP

中国农业科学 2007,40(11):2387-2394 Scientia Agricultura Sinica 利用基础导入系分析粳稻基因的遗传效应 陈庆全，穆俊祥，周红菊，余四斌 （华中农业大学作物遗传改良国家重点实验室/ 国家植物基因研究中心，武汉430070 ） 摘要: 【目的】为了有效地评价粳稻等位基因在籼稻遗传背景中的遗传效应，为籼粳亚种间优良基因的利用 提供理论和实践依据。【方法】以粳稻品种日本晴（Nipponbare）为供体亲本、籼稻品种珍汕97B（Zhenshan 97B） 为受体亲本，经过杂交、回交，结合分子标记辅助选择，构建了88个基础导入系, 每个导入系具有相同的籼稻遗 传背景，且只含有一个或少数粳稻的导入片段，每个导入系的导入片段叠加起来能覆盖整个粳稻的染色体。利用 基础导入系群体进行单株产量、有效分蘖数、千粒重和每穗实粒数的数量性状基因（QTLs）定位和遗传效应分 析。【结果】共检测到29个具有显著遗传效应的位点（QTLs）。其中6个QTL 具有正向的加性效应和超显性效应， 其余 23 个 QTL 具有负向的加性效应和超显性效应。【结论】一些粳稻基因导入到籼稻中能表现出不同程度的增产 效应；超显性效应对水稻籼粳杂种优势起重要作用。 关键词：水稻；籼粳杂交；基础导入系；数量性状位点；超显性 Genetic Effect of Japonica Alleles Detected in Indica Candidate Introgression Lines CHEN Qing-quan, MU Jun-xiang, ZHOU Hong-ju, YU Si-bin (National Key Laboratory of Crop Genetic Improvement and National Plant Gene Research Center in Wuhan, Huazhong Agricultural University, Wuhan 430070) Abstracts: 【Objective 】To accurately assess genetic effect of alleles fromjaponica in a genetic background of indica and to efficiently utilize inter-subspecific heterosis between indica and japonica . 【Method 】We have developed a set of novel population with 88 candidate introgression lines (ILs) through backcross program and marker-aided selection by using japonica variety Nipponbare as donor and indica variety Zhenshan97B as recurrent parent. Each of the ILs contains a single or few japonica introgression chromosomal segments within the uniform indica genetic background, and together all the introgression segments can cover the whole japonica genome. The genetic effects of each introgression segment in the 88 ILs were analyzed on yield and its component traits including productive tillers, grains per panicle and 1000-