一个谷子新抗锈基因的AFLP标记.docVIP

一个谷子新抗锈基因的AFLP标记 赵立强1,2，潘文嘉2，瓮巧云3，邢继红2，董志平1，董金皋21河北省农林科学院谷子，石家庄 050031；2河北农业大学生命科学学院，保定 071001；3河北北方学院，河北张家口 075131Identification of AFLP Markers Linked to a Novel Rust Resistance Gene in Foxtail Millet ZHAO Li-qiang1,2, PAN Wen-jia2, MA Ji-fang1, WENG Qiao-yun3, DONG Li1, QUAN Jian-zhang, XING Ji-hong2, DONG Zhi-ping1, DONG Jin-gao2 (1Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031; 2Life Science of College, Agricultural University of Hebei, Baoding 071001, Hebei; 3Hebei North University, Zhangjiakou 075131, Hebei) Abstract: 【Objective】 The AFLP makers linked to rust resistance gene in foxtail millet were studied as they were useful for new resistance genes and the rational usage of marker-assisted selection in millet resistance breeding. 【Method】 A cross between resistant cultivar “Shilixiang” and susceptible cultivar “Yugu 1” was used to map foxtail millet rust resistance gene(s). Based on authentication result of F1 and F2 progenies inoculated with the Uromyces setariae-italicae Yoshino strains 93-5, resistant and susceptible pools were established. With 128 EcoRⅠ/MseⅠ primer combinations, AFLP analysis was performed on parents and two bulks. AFLP makers were applied to construct a genetic linkage map of resistance gene in an F2 population of 131 individuals, which were sequenced and then converted to stable SCAR markers. 【Results】 The inoculation test showed that the resistance of “Shilixiang” to millet rust was controlled by one dominant gene. In 128 AFLP primer combinations, three markers were found to be linked to the rust resistance gene, with the genetic distance of 7.4cM、9.2 cM and 27.4 cM respectively, which was tentatively named Rusi1. A preliminary genetic linkage map was constructed using 3 AFLP markers. Furthermore, the three cloned fragments were sequenced and then E+CTT/M+TAC-256 was successfully converted to a stable SCAR marker. Genetic linkage map of foxtail millet rust resistance gene Rusi1 was