昆明裂腹鱼不同组织乳酸脱氢酶同工酶研究.pdfVIP

( ) 　贵州农业科学 　20 10 ,38 2 :140～14 1 　Guizhou A gricult ural Sciences ( ) [文章编号] 100 1360 1 20 10 020 1070 14002 昆明裂腹鱼不同组织乳酸脱氢酶同工酶研究 胡思玉 , 陈永祥 , 赵海涛 , 王 俊 (毕节学院 , 贵州 毕节 55 1700) 　　[ 摘 　要] 采用不连续聚丙烯酰胺凝胶电泳法 ,对昆明裂腹鱼的眼球 、心脏 、肾脏 、性腺 、脾脏 、肝胰脏 、 ( ) 脑 、鳃和肌肉等 9 种组织的乳酸脱氢酶同工酶 L D H 进行了测定 。结果表明: 昆明裂腹鱼 L D H 共有 15 条 酶带表现出特异性 ,在不同组织中检出的L D H 酶带数量不同 , 以肝胰脏中最多 ,共检出 9 条 ;在酶活性方 面 ,L D H 在脑 、鳃 、肌肉和肾脏等组织中活性最强 ,而在眼球组织中最弱 。 [ 关键词] 昆明裂腹鱼 ; 乳酸脱氢酶同工酶 ; 不连续聚丙烯酰胺凝胶电泳 [ 中图分类号] S965 . 199 [ 文献标识码] A Study on L act at e Dehydro gena se Iso enzyme in Differ ent Ti ssue s of Shizothor ax gr ahami HU Siyu , C HAN Yongxian g , ZHA O Hait ao , WAN G J un ( De p a r t ment o f Envi r onment a n d L i f e Sciences , Bij ie College , Bij ie , Guizhou 551700 , Chin a) 　　Abstract : Lactate dehydrogenase isoenzyme (LD H) in eye ,heart ,kidney ,gonad ,spleen ,liver pancreas ,brain , gill ,and mu scle of S hiz othorax g raham i was determined by discontinuous polyacrylamide gel electrop horesis. The result s showed t hat t here were total 15 enzymatic bands wit h t he sp ecificity in LD H of S hiz othorax g rahami , t here was different number of enzymatic bands in different tissues of S hiz othorax g raham i and total 9 enzymatic bands were determined in it s liver p ancreas. The LD H activity in it s brain ,gill , mu scle and kidney was t he highest and the LD H activity in it s eye was t he lowest in it s 9 tissues. Key words : S hiz ot horax g rah am i ; lact at e dehydro gena se i soenzyme