枇杷花药胚SDS-PAGE蛋白质电泳体系优化初探.pdfVIP

枇杷花药胚SDS蛋白质电泳体系优化初探 辜润智1，王永清1’+，秦红玫1一，吕秀兰1，邓群仙1，严娟1 (1四川农业大学园艺学院，四川雅安625014：2西华师范大学生命科学学院，四川南充637002) 摘要：以枇杷花药胚为材料，研究了不同蛋白提取方法，不同丙酮干粉处理方法，不同上样量，不同考马斯亮 表明：用改良丙酮沉淀法提取缝白，丙酮干粉溶于上样缓冲液后先煮沸再离心，上样量为25ul，采用过滤后的0．1％考 马斯亮蓝R-250震荡染色和含5％乙醇，10％醋酸的脱色液脱色最终SDS电泳效果最好，所得条带清楚。 关键词：花药胚；提取；SDS—PAGE电泳 A on of Study SDS—PAGE PreliminaryOptimization ElectrophoresisTechnique forProteinsofAnther-Derived in EmbryosLoquat GuRunzhil，Wang Hongmeil，2，Lv Ounxianl。Yan Yongqingl*，Qin Xiulanl，Deng Juanl of (1CollegeHorticulture，SichuanAgricultural University，Ya’an，Sichuan625014， ofLife WestNormal China；2CollegeScience，China 637002， China) anther—derivedwereusedto theeffects Abstract：Loquat embryos study ofthedifferentprotein extraction methods，differenttreatmentsofdryacetone，differentvolume，differentCoomassie sample brilliantblue R一250 SOlutionand onSDS—PAGE of staining 1iquidportfolio electrophoresis．Thesystem anther—derivedSDS—PAGE was BestSDS—PAGE loquat embryo proteinelectrophoresiSoptimized． resultwasobtainedwiththemodified extractofacetone electrophoresiS protein dissolvedin afterthefirst drypowder samplebuffer，boiled the of25ui： Centrifugation：USingsample filtered 1iant USing 0．1％Coomassiebril blueR一250shake andwith5shocksethan01．10％acetic staining acidsolutiondecoloration．InthiS thebandswereclear． way word