家蝇幼虫抗菌蛋白的抗菌特性及其组成.pdfVIP

中国媒介生物学及控制杂志2006 年 6 月第 17 卷第 3 期　Chin J Vector Bio Control ,J une 2006 ,Vol. 17 ,No. 3 1·91 · 文章编号 :1003 4692 (2006) 03 0191 03 　　 ·论著· 家蝇幼虫抗菌蛋白的抗菌特性及其组成 万启惠 ,刘晖 ,贺莉芳 ,张曦 　　【摘要】　目的 　观察不同诱导源诱导家蝇幼虫产生抗菌蛋白的抗菌特性及其蛋白组分。方法 　取 5 种诱导源诱导 后提取抗菌蛋白 ,用不同细菌进行抑菌实验 ,观察 5 种诱导源诱导组抗菌蛋白的抗菌特性 ;5 种诱导源诱导组抗菌蛋白 经 SDS 聚丙烯酰胺凝胶电泳 ,观察抗菌蛋白的组分变化。结果 　各诱导组产生的抗菌蛋白均能抗多种 G+ 菌、G- 菌和 耐药菌株 ,但对不同细菌的抗菌活性有所差别。各诱导组抗菌蛋白中有 2 条电泳带与对照组完全相同 ,但含量高于对照 组 ;各诱导组与对照组比较均出现不同的新的蛋白带。结论 　家蝇幼虫诱导后产生的抗菌蛋白抗菌谱广 ;不同诱导源可 诱导家蝇幼虫产生相同蛋白及不同的新蛋白 。 【关键词】　家蝇幼虫 ; 抗菌蛋白 ; 诱导源 ; 抗菌活性 ; 电泳分析 　　中图分类号 :R384. 2 ; R978. 1 + 6 　　　　文献标识码 :A Antibacterial Characterization and Component of the Antibacterial Proteins in Musca domestica Larva by Different Inducements 　 W A N Qihui , L IU Hui , HE L if an g , ZHA N G Xi . Depart ment of Parasitology , Zunyi Medical College , Zunyi 563003 , China 【Abstract 】　Objective 　To observe the antibacterial characterization and component of the antibacterial proteins in the M usca domestica larva by different inducements. Methods 　Several different bacteria were used as detecting index to detect the antibacterial activity of antibacterial proteins which is induced by five inducements. SDSPA GE was used as detecting index to observe the proteins compared with the control group. Results 　These antibacterial proteins could inhabit many bacteria (including some Gramepositive , Gramenegative bacteria and rifampin resistant E . coli ) , but the antibacterial activities were different. The antibacterial proteins induced by five inducements had two same component compared with the control group , but their content were different ,and di