变性梯度凝胶电泳法研究我国不同土壤氨氧化细菌群落组成及活性_袁飞.pdfVIP

25 6 Vol. 25, No. 6 2005 6 ACT A ECOLOGICA SINICA Jun. , 2005 袁　飞, 冉　炜, 胡　江, 沈其荣* ( , 210095) : 3 , ( DGGE) ( ) 28 , , 100% AOB d ; , 4. 9% 3 , 3 , 3 ( NOB) amoA ( am monia monooxygenase) PCR DGGE 4 , 4 , , N itrososp ira cluster3bz97838 N itrososp ira cluster3aAF353263 5 , , , , Nitrososp ira cluster 3b Z97849 4 , , , , 3 353263 3 , N itrososp ira cluster a AF amoA PCR DGGE 16S rDNA PCR DGGE ; 3 , , pH ; 3 , 3 , : ; ; ; : 1000 0933( 2005) 06 1318 07: S154. 1, S 154. 37: A - Ammonia oxidizing bacteria communities and their influence on the nitrification potential of Chinese soils measured by denaturing gradient gel electrophoresis ( DGGE) * ( , YUAN Fei, RAN Wei, HU Jiang , SHEN Qi Rong College of N atural Resources and Environmental Science N anj ing A gr icultural University , N anj ing 210095, China) .Act a Ecol og ica Sinica , 2 5, 25( 6) : Abstract N itrif ication is an import ant com ponent of the global nit rogen cycle and the end product is nitrate and t he rate of: nitrate production is very different among soil types N itrif ication can lead to different effect s ranging from too much nitrate. , causing environmental problems, or too little nitrate can lead to reduced crop yields. Differences in physical and chemical properties of soils are often w ell documented, but much less is know n about the soil nitrifying bacterial communities. We : ( : 2004 03 23; : 2004 09 19 : ( 1974) , , , , . * Author for correspondence. E mail: shen qirong@ njau . edu. cn Foundation item: National Natural Science Foun