原生质体融合选育乳清发酵生产燃料乙醇的酵母菌种.pdfVIP

年第 期（总第 期）· ) 28 酿酒科技 2009 11 185 LIQUOR－MAKING SCIENCE ＆ TECHNOLOGY 2009 No．11(Tol．185 原生质体融合选育乳清发酵生产燃料乙醇的酵母菌种 王瑞生，肖冬光，郭学武，张翠英，马文强 （工业微生物教育部重点实验室，天津科技大学生物工程学院，天津 300457 ） 摘 要：以酿酒酵母AY-5 和克鲁维酵母TY-13 为出发菌株进行原生质体融合选育乳清发酵优良菌种。通过对 原生质体融合条件的优化获得152 株初筛菌株，经麦芽糖和乳糖杜氏管试验复筛得4 株融合子。对4 株融合子进行 乳清发酵实验，从中选出1 株发酵优良的菌株R-1，与亲本比较，发酵速度明显提高。 关键词：微生物；乳清；燃料乙醇；原生质体融合 ： ； ； ： ： （ ） 中图分类号 TQ93-3 TQ813 TS262.2 文献标识码 A 文章编号 1001－9286 2009 11－0028－03 A Recombinant Saccharomyces cerevisiae Strain for Efficient Conversion of Lactose in Cheese Whey into Ethanol WANG Rui-sheng, XIAO Dong-guang, GUO Xue-wu, ZHANG Cui-ying and MA Wen-qiang （Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China ） Abstract: Intergeneric protoplast fusion between Saccharomyces cerevisiae AY-5 and Kluyveromyces marxianus TY-13 was carried out to obtain quality strains for lactose fermentation in whey. 152 fusants were selected from the fusion plates. Among these fusants, 4 strains were finally ob- tained through maltose and lactose Durham test. Then the 4 fusants were used for whey fermentation experiments and strain R-1 with satisfactory fermentation performance was selected finally. Compared with parent strains, strain R-1 had quicker fermentation speed. Key words: microbe; whey; fuel ethanol; protoplast fusion 乳清是生产干酪及干酪素时产生的副产物， 具有很 1.1.1 菌种 [1] 高的生物学价值与良好的功能特性 。 目前，