甘蓝型油菜显性核不育基因及与恢复基因的等位性分析.pdfVIP

2006,39(3):456-462 Scientia Agricultura Sinica 1 2 ( / 430070 330200) Allelism Analysis of Dominant Genic Male Sterility Gene and Its Restorer Gene in Brassica napus 1,2 1 1 1 1 SONG Lai-qiang , FU Ting-dong , YANG Guang-sheng , TU Jin-xing , MA Chao-zhi 1 ( National Key Laboratory of Crop Genetic Improvement, National Sub-center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070 ; 2Jiangxi Academy of Agricultural Sciences, Nanchang 330200) Abstract: Objective Dominant genic male sterility (DGMS) in Brassica napus is gaining importance in breeding program and utilization of heterosis. The multiple allele inheritance model has been characterized recently by analyzing the allelism between the male sterility allele (Ms) in 609A and the fertility restoration allele (Mf) in 609B as well as the alleles from several restorers tested. The present study was designated to provide further validation of the result with more restorers and another DGMS line. Method Extensively screening was made through crossing 609A with parental materials, and the restored F1 s were subjected to allelism analysis with temporary maintainer test methodology. The fertile plants in Rs1046AB were crossed with temporary maintainers and the fertile plants in the subsequent progenies were selected to be selfed and backcrossed. The analysis of allelism between the two sterile lines was completed through testing the allelism between the Ms in 609A and the Mf in Rs1046B. Result Twenty eight restorers for DGMS