库尔勒香梨_60_Co_辐射育种材料的过氧化物同工酶研究.pdfVIP

20 1 V o .l 20 N o. 1 2008 3 Jou rn al of T armi U n iversity M ar. 2008 : 1009- 0568 2008) 01- 0006- 04 60 Co- C ¹ 1, 2 1, 2 2 1 1 1 , , 843300) 2 , 843300) , : , R f 0. 46, ) , ) ; 60Co- C; : S 60 3. 6 : A Studies on P eroxidase Isoenzym e of the Kuerle A rom a P ear by 60Co- CRay R adiation 1, 2 1, 2 2 1 1 W ang X in jian W u Cu iyun B ian M e ina Jin Q iang Y ang Y u 1 key laboratory of P rotect ion and U tilization of B io log ica l Resources in T armi Basin of X in jiang P roduction C onstruction C rop s, A lar, X in jiang 843300) 2 Co llege of P lant Sc ience and T echno logy, T armi U niversity, A lar, X in jiang 843300) A bstrac t A dopted the d iscontinuou s and perpend icular flat panel po lyacry lam ide g el e lec trophresis, the perox idase isoenzym e in the slice o f leaves o f K uerle A rom a P ear processed w ith different am ount of rad ia tion w ere ana ly zed. T he result show ed that the bands quan- tity and enzyme activ ity w ere ma in ly d ifferent. T he predom inant band s R f of a ll samp les w as 0. 46, w hich cou ld be deem ed the stab le characteristic enzym e o f