中国淋巴囊肿病毒胸苷酸合酶基因结构特点及分析.pdfVIP

维普资讯 第 l9卷 6期 中 国 病 毒 学 l9 (6)：602—606 2004年 11月 VIROLoGICA SDiICA November 2004 中国淋巴囊肿病毒胸苷酸合酶基因结构特点及分析 赵 哲 2，张奇亚 (1．中国科学院水生生物研 究所 ，淡水生态与生物技术国家重 点实验室，湖北武汉 430072：2．中国科学院研究生院，北京 100039) StructureAnalysisofThymidylateSynthaseGenefrom LCDV-C ZHAOZhe，ZHANGQi．ya (j．StateKeyLaboratoryofFreshwaterEcologyandBiotechnology，InstituteofHydrobiology，ChineseAcademyof Sciences。Wuhan430072，China；2．Graduateschool,ChineseAcademyofSciences，Beijing100039,China) Abstract：ThymidylateSynthase(TS)isanenzymeinvolvedinmakingoneofthemajorbuilding blocksforDNAdeoxythymidylicacid(dTMP)，andplaysacriticalroleincellgrowthanddivisionand isoverexpressedinthemajorityofcancers．Lymphocystisdiseasevirus(LCDV)belongtoiridoviridae， andthecausativeagentoflymphocystisdisease，whichhasbeenreportedtooccurinover100different fish speciesworldwide．and viralinfection thatcausescellstobecomemegaloblastic，thusforming smalltumorsrbumpsorgrowths)．ThecompletenucleotidesequenceoftheLCDV—C genomewas recentlydetermined．Amongthesequencedvertebrateiridoviurses．1ymphocystisdiseaseviursisolated fromChina(LCDV-C)isofthelargestgenome．Computer-assistedanalysisrevealedthepotentialopen reading rfamesORF 0l1L 0fLCDV．C wasTS gene．thefirstreport ofcompleteTS genefrom vertebrateiridoviurses．TheLCDV．CTSgeneconsistsof858bpinlenght withabasecompositionof 28．2％ G+C．andencodedaproteinof286arninoacidswithapredictedsizeof32．7KD．Comparisonof hteLCD、，．CTSt0TSfrom Chiloiridescentvirus(CIV)，Ambystomatigrinum viurs(rV)andtiger rfogviursfTF )threeiridoviursesandothersixteenspecies，showedextensiveaminoacidsimilarities OfTSbetweenfouriridovirusesandotherspecies．Thepredict