茶多酚对假单胞菌抑菌机理研究(电导率,蛋白,AKP).pdfVIP

20 10年第 1期 　产品开发 ·试验研究　 M EA T INDU STRY 总第 345期 茶多酚对假单胞菌抑菌机理研究 孙京新 　王文娟 　青岛农业大学食品学院　山东青岛　266 109 　　摘 　要 　以假单胞菌为研究对象 ,考察茶多酚的抑菌作用及其抑菌机理 。首先通过平板法确定茶多酚对假单 胞菌最小抑菌浓度 。然后采用滤纸片法确定不同浓度的茶多酚对假单胞菌的抑菌效果 。通过生化检测研究茶多酚 对假单胞菌的抑菌机理 。结果表明:茶多酚对假单胞菌最小抑菌浓度为 0. 3% ;茶多酚对假单胞菌有很强的抑菌作 用 ;当茶多酚作用于菌体细胞后 ,能够逐步破坏其细胞壁的完整性 ,使得碱性磷酸酶渗出 ,继而使细胞膜的通透性增 加 ,导致金属离子 、蛋白质的渗漏使细胞代谢发生紊乱 ,逐渐破坏细胞结构 ,从而起到抑菌作用 。 关键词 　茶多酚 　假单胞菌 　抑菌活性 　抑菌机理 An tim icrob ia l action m echan ism of tea polyphenols on p seudom onad A b stract　A ction m echan ism and an tim icrob ial effect of tea po lyp heno ls again st the p seudomonad were stud ied. The m in im um inh ib itory concen tration of tea po lyp heno ls again st the p seudomonad wa s deter m ined through the p late m ethod and then the an tim icrob ial effects of varying concen tration of tea po lyp he no ls again st the p seudomonad were e stab lished through the film filter p ap er. A dop ting b io logical and chem ical te sting, the an tim icrob ial m echan ism of the tea po lyheno ls again st the p seudomonad wa s studied. A nd the re su lts revealed that the m in im um inh ib itory concen tration of tea po lyp heno ls again st the p seudomonad wa s 0. 3% , and tea po lyp heno ls had a very strong inh ib ition effect on the p seudomonad. The comp letene ss of cytoderm wa s de stroyed in the p re sence of tea po lyp heno ls, wh ich cau sed tran sudation of alkaline p ho s p hata se, then the p erm eab ility of bacterial cell m emb rane wa s increa sed ( increa se the leakage of p rotein and sugar) and the cytoarch itectu re wa s de stroyed gradually that wa s the an tim icrob ial m echan ism