荧光假单胞菌phlD基因的克隆_表达及功能.pdfVIP

29 5 Vol. 29 No. 5 2009 5 Tra sact io s of Beiji g I st itut e of Tech ology May 2009 p hlD 高海军, 肖丹, 杨永政, 高芳芳, 庞思平 ( , 100081) : 2 , 4-( 2 , 4-DA PG) phlD , E. coli BL21 ( DE3) /p ET28a (+ ) phlD , PhlD 42 000 Da . PhlD P K S Ó, PhlD A . . : phlD ; ; ; : Q932 : A : 1001-0 45 (2009) 05-04 5-0 Cloning, Expression and Functioning of p hlD Genes from Pseudomonas Fl uorescens GAO Ha-i ju , XIAO da , YANG Yo g-zhe g, GAO Fa g-fa g, PANG S-i pi g ( School of Life Scie ce a d Tech ology , Beiji g I st itute of Tech ology , Beiji g 100081 , Chi a) Abstract: T he critical ge e p hlD for 2 , 4-DAPG biosy thesis from Pseudomonasf luorescens w as clo ed a d expressed i E . coli BL21 ( DE3) / pET 28 a( + ) /p hlD recombi a t strai . The ge e of p hlD w as amplified usi g Pseudomonas f luorescens 2 P24 ge ome as template. T he PhlD of 42 000 Da fusio protei w as i duced a d expressed i E. coli BL21 ( DE3) / pET28 a( + ) system. T he p hlD ge e seque ce w as a alyzed, the se ior structure of the protei coded by the p hlD also forecasted. PhlD was o e of polyketide sy thase of type- Ó, a d the fu ctio of PhlD w as similar to the polyketal sy thase a d acyltra sferase. T he ferme tatio liquid of recombi a t strai w as detected, a d sy thesis of the phlorogluci ol w as impleme ted. Key words: p hlD ge e; clo i g; recombi a t bacteria; phlorogluci ol , phlACBD , phlD 2, 4-DA PG , , , P hlD : (2-MA PG) 2, 4- A , (2, 4-DAP G) phlD , [ 1] phlACBD 2, 4-DAP G[ 2] . . 2, 4-DA P G ,