CdTe纳米晶复合薄膜的制备和其在生物荧光标记方面的应用研究.pdfVIP

CdTe纳米晶复合薄膜的制备及其 在生物荧光标记方面的应用研究 朱建淳 田雨辰甘礼华 刘明贤孙 颖 徐子颉郝志显 朱大章陈龙武 同济大学化学系，上海200092 摘要：以巯基丙酸为稳定剂，用水相法合成了CdTe纳米晶。通过CdTe纳米晶与 阳离子聚电解质聚二烯丙基二甲基氯化铵(PDDA)和阴离子聚电解质聚苯乙烯磺酸钠 (PSS)之间的静电相互作用，在聚苯乙烯微球(PS)表面通过层层静电自组装方法制备 了多层CdTe纳米复合薄膜。透射电镜、紫外-可见光谱和荧光光谱研究结果表明，所得 CdTe纳米晶粒径分布比较均匀，平均粒径约为5nm，且具有很高的荧光光致发光性。 以所得CdTe纳米晶/PS荧光复合微球为标记物，对荧光素标记羊抗兔(Goatanti- RbIgG/HTC)和纯化兔血清(RabbitIgG)分别进行了荧光标记，并研究了CdTe纳米晶 /PS荧光复合微球表面RabbitIgG和Goatanti-RbIgG/FITC之间的免疫反应。 关键词：CdTe纳米晶；复合薄膜；制备；自组装；生物荧光标记；免疫反应 PreparationofCdTeNanocompositeFilms andItsApplicationinBiologicalLabeling ZHUJianchunTIANYuchenGANLihua LIUMingxian SUNYingXUZijieHAOZhixian,ZHUDazhangCHENLongwu DepartmentofChemistry,TongjiUniversity,Shanghai200092,China Abstract:CdTenanocrystalswerepreparedinaqueousphasebyusing3- mercaptopropionicacidasstabilizer.Throughtheelectrostaticinteractionsamongthe negativesurfacechargesonCdTenanocrystals, thecationicgroupsonpoly (diallyldimethylammoniumchloride)andtheanionicgroupsonpoly(sodium4-styrene sulfonate),CdTecompositenanofilrnswerefabricatedonthesurfaceofpolystyrene microspheres(PS) bylayer-by-layerelectrostaticself-assemblymethod. The characterization of transmission electron microscopy, UV-Vis spectra and photoluminescencespectrashowthattheresultantCdTenanocrystalshaveanarrow sizedistributionwithaveragesizesofabout5nmandshowhighluminescence intensity.Goatanti-RbIgG/FITCandRabbitIgGwerelabeledbytheCdTe nanocrystals/PSmierospheres.TheimmunoreactionbetweenRabbitIgGandGoat anti-RbIgG/FITConthesurfaceofPSmicrospherescoatedwithCdTenanocrystals wasalsoinvestigated. Keywords:CdTenanocrystals; compositefilm; preparation; self-assembly; biologicallabeling;immunoreaction 球为载体， 廷得了CAT 受基在其催卅 ：体或抗原与 凸分离，接着j tanti-RbIg( i鬻鬻 ≯鬻 麓落 ’e纳米晶白 买越结果 }ITC发生 光激发峰 Z业：哎刊 结果考 。anti-] YYdlng and applic