苹果汁中耐热菌生长与代谢动力学研究.docVIP

苹果汁中耐热菌生长与代谢动力学研究 摘 要 我国是世界苹果生产大国，亦为世界苹果浓缩汁生产第一大国，但长期以来，以耐热菌为代表的安全性问题成为我国苹果浓缩汁产业国际化发展的技术瓶颈，突破此技术瓶颈的关键是建立有效的控制技术体系，本文以浓缩苹果汁中耐热菌的控制为目标，以标准耐热菌菌株和分离耐热菌菌株为试验材料，通过对耐热菌培养基的优化、耐热菌细胞生长动力学、耐热菌代谢产物的HS-SPME-GC/MS检测技术方法、耐热菌代谢动力学的系统试验研究，旨在为建立适合我国国情的耐热菌控制技术体系奠定理论基础。 其主要研究结果如下： 1、本试验室分离获得的耐热菌菌落形态与显微形态与标准菌株基本一致； 2、优化出的标准菌株和分离菌株培养基基本一致，其组成为：标准耐热菌培养基：MgSO4·7H2O 1 g/L，CaCL2·2H2O 0.5g/L， KH2PO4 1.2g /L、MnSO4·4H2O，0.5g/L，蒸馏水1L，蛋白胨5 g /L，果糖2 g /L，酵母粉2 g /L。分离耐热菌培养基：MgSO4 ·7H2O， 1 g/L，CaCL2·2H2O 0.5g/L， KH2PO4 1.2g /L，MnSO4·4H 2O，0.5g/L，蒸馏水1L，蛋白胨5g/L，葡萄糖2g/L，酵母粉2g/L。 3、建立了标准耐热菌株和分离耐热菌株在苹果汁培养基、优化培养基中吸光度与活菌数关系模型； 4、运用积分变换建立了标准菌与分离菌在苹果汁培养基、优化培养基中生长动力学模型； 5、建立了耐热菌代谢产物的顶空固相微萃取-气相色谱/质谱联用(HS-SPME-GC/MS)检测技术方法与技术条件：①检测耐热菌代谢产物-愈创木酚和2，6-二溴苯酚适宜萃取纤维头为DVB/CAR/PDMS；②其最佳检测条件为：选择55℃作为萃取温度，萃取时间30min，加入Na2SO4效果最好，产物解析时间5min. 6、应用HS-SPME-GC/MS检测标准菌与分离菌代谢产物,获得耐热菌的代谢产物生成曲线。 7、确定了标准菌与分离菌在苹果汁培养基、优化培养基中代谢动力学模型，模型能够很好的模拟代谢产物的生成过程。 关键词： 苹果汁；耐热菌；HS-SPME-GC/MS；生长与代谢；动力学模型 STUDY ON GROWTH DYNAMICS ?AND PHARMACOKINETICS OF ALICYCLOBACILLUS ACIDOTERRESTRIS IN Apple juice ABSTRACT China is the biggest apple production country and the biggest apple juice concentration production country in the world.However, alicyclobacillus acidoterrestris problem has become the biggest technique problem in the production of apple juice concentration for a long time. The key to break through the technical bottleneck is constructing technical standards system efficiently.In this paper,two alicyclobacillus acidoterrestris strains were selected as experimental material.The study were that the optimization of culture medium, confirm the growth dynamics models,the optimization of measure condition about guaiacol and 2，6-dibromophenol used HS-SPME-GC/MS, confirm the pharmacokinetics models between the growth and guaiacol and 2，6-dibromophenol in difference culture medium. The control theory system was established for the alicyclobacillus acidoterrestris in china. The main results in this paper showed: 1、 The colony’s shape and micro morphol