DETECTIONOFGENEEXPRESSIONOFSERINE：的丝氨酸基因表达的检测.pdfVIP

Iraqi J. Biotech.11 (2):340-348 (2012) Hayder Z. Ali DETECTION OF GENE EXPRESSION OF SERINE PALMITOYLTRANSFERASE (SPT2) IN MOUSE CELL LINE RAW264.7 INFECTED WITH LEISHMANIA MEXICANA AMASTIGOTES Hayder Z. Ali Dept. of Biology, College of Science, University of Baghdad. ABSTRACT Leishmania species are the causative agent of a tropical disease known as leishmaniasis. Previous studies on the old world species Leishmania maj or, showed that the amastigotes form which resides inside the macrophage of the vertebrate host, utilize host’s sphingolipids for survival and proliferation. In this study, gene expression of serine palmitoyltransferase (SPT) subunit two (MmL CB2) of the mouse macrophage cell line (RAW264.7), which is the first enzyme in the de novo sphingolipid biosynthesis, was detected in both infected and non-infected macrophages. This was detected under condition where available sphingolipid was reduced, with the new world species Leishmania mexicana. Results of qPCR analysis showed that there was no difference in the expression of MmL CB2 in infected and non-infected macrophages, under normal and serum-reduced media, suggesting that host sphingolipid did not up-regulated during infection. This can be concluded as a difference between the Old and New world Leishmania on the level of host-parasite interaction. Key words: Leishmania mexicana, SPT, RAW264.7, myriocin. *To whom correspondence should be addressed (E-mail: h.z.ali@scbaghdad.ed.iq) 340 Iraqi J. Biotech.11 (2):340-348 (2012) Hayder Z. Ali (SP T2) مﯾزﻧﻹ ﻧﯾﺟﻟا رﯾﺑﻌﺗﻟا نﻋ يرﺣﺗﻟا