RecombinantHumanIL-7Interleukin-7Protein,HEK293：重组人白细胞介素-7蛋白表达IL-7，.pdfVIP

Recombinant Human IL‐7 /Interleukin‐7 Protein, HEK293 Expressed Catalog # IL7‐H4219 For Research and Further Cell Culture Manufacturing Use Description Source Recombinant Human IL-7 /Interleukin-7 Protein, HEK293 Expressed (rh IL7) Asp 26 - His 177 (Accession # AAH47698) was produced in human 293 cells (HEK293) at ACROBiosystems. Predicted N‐terminus Asp 26 Molecular rh IL7 contains no tag, and has a calculated MW of 17 kDa. The predicted N-terminus is Asp 26. DTT-reduced Protein Characterization migrates as 24 kDa and 26 kDa in SDSdue to different glycosylation. Endotoxin Less than 1.0 EU per μg of the rh IL7 by the LAL method. Purity 95% as determined by SDS. Bioactivity The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 2E8 cells. The ED50 0.2 ng/mL, corresponding to a specific activity of 5X106 Unit/mg. Formulation and Storage Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. Contact us for customized product form or formulation. Reconstitution See Certificate of Analysis for reconstitution instructions and specific concentrations. Storage Lyophilized Protein should be stored at -20℃ or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20℃ or -70℃. Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: ● 4-8℃ for 12 months in lyophilized state; ● -70℃ for 3 months under