STA-321-dna-double-strand-break-assaypdf：sta-321-dna-double-strand-break-assaypdf.pdfVIP

Product Manual OxiSelect™ DNA Double Strand Break (DSB) Staining Kit Catalog Number STA-321 100 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction DNA double-strand breaks (DSBs) are probably the most dangerous of the many different types of DNA damage that occur within the cell. DSBs are generated by exogenous agents such as ionizing radiation (IR) or by endogenously generated reactive oxygen species and occur as intermediates during meiotic and V(D)J recombination. A very early step in the cellular response to DSBs is the phosphorylation of a histone H2A variant, H2AX, at the sites of DNA damage. H2AX is rapidly phosphorylated (within seconds) at serine 139 when DSBs are introduced into mammalian cells resulting in discrete γ-H2AX (phosphorylated H2AX) foci at the DNA damage sites. H2AX phosphorylation also appears to be a general cellular response to processes involving DSB intermediates including V(D)J recombination in lymphoid cells and meiotic recombination in mice. Phosphorylation of H2A at serine 139 causes chromatin decondensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the sites of DNA damage. Cell Biolabs’ OxiSelect™ DNA DSB Staining Kit is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates. The kit provides sufficient reagents for up to 100 stainings in 96- well plate. Related Products 1. STA-320: OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) 2. STA-324: OxiSelect™ Oxidative DNA Damage Quantitation Kit (AP sites) 3. STA-325: OxiSelect™ Oxidative RNA Damage ELISA Kit (8-OHG Quantitation) 4. STA-350: OxiSelect™ Comet Assay Kit (3-Well Slides), 15 Assays 5. STA-351: OxiSelect™ Comet Assay Kit (3-Well Slides), 75 Assays 6. STA-352: OxiSelect™ Comet Assay Slides (3-Well