刺参酸性黏多糖对HepA荷瘤小鼠的增效作用及其机制研究.pdfVIP

2010 21 12 LISH IZHEN MEDICINE AND MATERIA MEDICA RESEARCH 2010 VOL. 21NO. 12 Hep A 赵秀梅, 冯文茹, 高 巍, 胡人杰* (, 300020) : 探讨刺参酸性黏多糖( SJAM P) 对化疗药物 ( CTXDDP5- F ) 的增效作用, 并初步对其作用机制进行研究 通过建立小鼠H ep A 实体瘤模型, 观察 SJAM P 与化疗药合用对肿瘤生长的影响采用高效液相色谱( HPLC)法, 观 察单用氟尿嘧啶及与 SJAM P合用在肿瘤组织中的药物浓度变化 在相同的剂量下, 化疗药物与 SJAM P 合用组和 单独使用化疗药组相比, 小鼠肿瘤的体积明显减小, 低剂量合用组已经接近或超过单独使用化疗药的中剂量组的抑瘤 率, 而且中剂量合用组的抑瘤率与高剂量组的抑瘤率相比也非常接近H PLC 测定结果显示, 与 SJAMP 合用肿瘤组织内 5- F 的含量低于单独给予氟尿嘧啶 SJAMP 具有广谱增效作用 : 刺参酸性黏多糖; 肿瘤; 增效; 高效液相色谱法 DO I: do:i 10. 3969/ j. issn. 2010. 12. 012 : R285. 5 : A : 2010) SynergisticEffectofAcidMucopolysaccharide inS tichop us j ap on icus Selenka onHepA in mice and itsm echanism ZHAO X i me,i FENG W enr , GAO W e,i HU Renjie (T ianj in Institute of M ed ical and P harm acolog ical Science, T ianj in 300020, ch ina ) Abstract: Objective To investigate the synergistic effect of acid m copolysaccharide inS t ichop us j ap on icus Selenka( SJAMP) in comb inationw ith chemotherape tics ( CTX, DDP, 5- F ) and itsm echanism. Methods TheH ep A m o se t m orm odelw as es tab lished to observe the t m or grow th when SJAMP w as sed in comb ination w ith chem otherape tics; the concentration of 5- F in sit ation alone orw ith SJAMP in the t m or was m eas red by H PLC. Results The t mor vol m es in m ice coadm inistered w ith SJAMP and chem otherape ticsw ere obvio sly sm aller than thosew ith chem otherape tics alone nder the sam e concen tration; the inh ibition rate of t m or grow th( IR ) in low dose com ed ication gro p was smi ilar to, even greater than that in m edi m dose antican cer agents gro p, and the IR in m edi m dose com ed ication gro p w as q ite smi ilar to that in h igh dose anticancer agents g