噻唑蓝法检测中药黄芪等复方制剂对人白血病细胞的影响_王敬毅.pdfVIP

# 1764# 辽宁中医杂志 2009年第 36 卷第 10期 1 1 1 1 1 1 2 , , , , , , ( 11 山东中医药大学附属医院血液科, 山东 济南 250011; 21 济南钢铁集团总医院外二科, 山东 济南 250101) : 目的: 运用噻唑蓝 (MTT)法检测 3种不同浓度中药黄芪复方药液对人白血病细胞的影响方法: 离人白 血病骨髓单 个核细胞, MTT 法检测不同浓度中药黄芪复方药液对细胞生长的影响结果: 浓度为 1g /mL的药液与 015g/ mL115g/mL 药液比较差异均有统计学意义(P 01 05)浓度 01 5g /mL与 11 5g /mL 药液比较差异无统计学意义结论: 中药黄芪药液对人白血病细胞体外生长抑制的影响不与药物浓度成正比 : 白血病; MTT; 中药黄芪复方 : R733. 7 : B : 1000- 1719( 2009) 10- 1764- 02 MTT Study on theResponse ofHuman LeukemicCell to TraditionalChineseRecipes ofHuangqi 1 1 1 1 1 1 2 WANG J ing-yi,X URui-rong , ZHOU Yan-f eng , LIUZhao-xia , CUI X ing , WANG Yan ,HAO Jian-guo (11 ep artment of Blood Intern-medicine, the Aff ilia TedH osp ital, Shandong TranditionalM edicine University, Jinan, 250011, Shandong, China; 21GenHosp of Jinan Iron and SteelGroup, Jinan 250011, Shandong, China) Abstract:Objective: To eterm ine the sensitivity of Leukem ic Cell to the three ifferent concentrations of tran itional m e -i cine fomu lar ofHuangq i byMTT assay.M ethods: Peripheralm arrow m ononuclear cells ( BMMC) from hum an leukem ic bone mar- row by Ficol.l To eterm ine the sensitivity of Leukem ic Cell to the three ifferent concentrations of tran itionalm e icine fomu lar of Huangq i by MTT assay. Results: 1g/mL concentration of the liqu i an 01 5g /mL, 11 5g /mL liqu i ifferences w ere statistically significant comparison (P 0105) . There w as no sign ificant ifference betw een the 015g/mL an the 11 5g /mL. Conclusion: The effects of the ligui on the hum an leukem ia cells in vitro grow th inh ib itionw ere non-pro