延髓腹外侧一氧化氮介导电针内关对急性心肌缺血大鼠心功能的作用.pdfVIP

LU Juan-Xiu et al: Medullary NO and EA on AMI 503 Acta Physiologica Sinica, August 25, 2004, 56 (4): 503-508 Research Paper Medullary ventrolateral nitric oxide mediates the cardiac effect of electroacupuncture at “Neiguan” acupoint on acute myocardial ischemia in rats 2 * LU Juan-Xiu , ZHOU Pei-Hua , WANG Jin, LI Xia, CAO Yin-Xiang, ZHOU Xu , ZHU Da-Nian Department of Physiology Pathophysiology, Shanghai Medical College of Fudan University, Shanghai 200032, China Abstract: Experiments were performed on male Sprague-Dawley (SD) rats anesthetized with a mixture of urethane and chloralose. A rat model of acute myocardial ischemia (AMI) was made by ligation of the left anterior descending branch of the coronary artery (LAD). After the LAD ligation, the ischemia area of the left ventricular wall became somewhat pale immediately. Under a light microscope, the pathological examination revealed that all the cells were swollen and in red color when the cardiac section was stained with hematoxylin basic fuchsin picric acid (HBFP), which indicated a typical change in the myocardial ischemia. In the AMI model, it was found that cardiac functions were markedly attenuated, such as decreases in the heart rate (HR), mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), maximal rate for left ventricular pressure rising and declining (±dp/dt ), velocity of contractile element ( V ) max CE and total area of cardiac force loop (L0), and an increase in the left ventricular end diastolic pressure (LVEDP). In such AMI rats, application of electroacupuncture (EA)